Role of Promoters and Micro RNA Backbone for Efficient Gene Silencing

Feng Li, Ram I. Mahato

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Promoters are regulatory elements that provide precise control of magnitude, specificity and duration of shRNA expression and gene silencing. The selection of a suitable promoter is therefore critical in the design of a shRNA expression system. We discuss the use of different promoters for shRNA expression, including constitutive promoters, inducible promoters, and tissue-/tumor-specific promoters. Recently, miRNA-based shRNAs have received increasing attention as a promising RNAi approach. We have therefore also summarized the use of a miRNA backbone to enhance gene silencing and to reduce toxicities. The application of miRNA-shRNA for combinatorial gene therapy is also discussed.

Original languageEnglish (US)
Title of host publicationAdvanced Delivery and Therapeutic Applications of RNAi
PublisherJohn Wiley and Sons
Pages83-108
Number of pages26
ISBN (Print)9781119976868
DOIs
StatePublished - Apr 26 2013

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Keywords

  • Cell-specific promoters
  • Combinatorial RNAi
  • Constitutive promoters
  • Inducible promoters
  • MiRNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Li, F., & Mahato, R. I. (2013). Role of Promoters and Micro RNA Backbone for Efficient Gene Silencing. In Advanced Delivery and Therapeutic Applications of RNAi (pp. 83-108). John Wiley and Sons. https://doi.org/10.1002/9781118610749.ch5