Role of Promoters and Micro RNA Backbone for Efficient Gene Silencing

Feng Li, Ram I Mahato

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Promoters are regulatory elements that provide precise control of magnitude, specificity and duration of shRNA expression and gene silencing. The selection of a suitable promoter is therefore critical in the design of a shRNA expression system. We discuss the use of different promoters for shRNA expression, including constitutive promoters, inducible promoters, and tissue-/tumor-specific promoters. Recently, miRNA-based shRNAs have received increasing attention as a promising RNAi approach. We have therefore also summarized the use of a miRNA backbone to enhance gene silencing and to reduce toxicities. The application of miRNA-shRNA for combinatorial gene therapy is also discussed.

Original languageEnglish (US)
Title of host publicationAdvanced Delivery and Therapeutic Applications of RNAi
PublisherJohn Wiley and Sons
Pages83-108
Number of pages26
ISBN (Print)9781119976868
DOIs
StatePublished - Apr 26 2013

Fingerprint

Gene Silencing
MicroRNAs
Small Interfering RNA
Genes
Gene therapy
RNA Interference
Genetic Therapy
Carcinogens
Toxicity
Tumors
Tissue

Keywords

  • Cell-specific promoters
  • Combinatorial RNAi
  • Constitutive promoters
  • Inducible promoters
  • MiRNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Li, F., & Mahato, R. I. (2013). Role of Promoters and Micro RNA Backbone for Efficient Gene Silencing. In Advanced Delivery and Therapeutic Applications of RNAi (pp. 83-108). John Wiley and Sons. https://doi.org/10.1002/9781118610749.ch5

Role of Promoters and Micro RNA Backbone for Efficient Gene Silencing. / Li, Feng; Mahato, Ram I.

Advanced Delivery and Therapeutic Applications of RNAi. John Wiley and Sons, 2013. p. 83-108.

Research output: Chapter in Book/Report/Conference proceedingChapter

Li, F & Mahato, RI 2013, Role of Promoters and Micro RNA Backbone for Efficient Gene Silencing. in Advanced Delivery and Therapeutic Applications of RNAi. John Wiley and Sons, pp. 83-108. https://doi.org/10.1002/9781118610749.ch5
Li F, Mahato RI. Role of Promoters and Micro RNA Backbone for Efficient Gene Silencing. In Advanced Delivery and Therapeutic Applications of RNAi. John Wiley and Sons. 2013. p. 83-108 https://doi.org/10.1002/9781118610749.ch5
Li, Feng ; Mahato, Ram I. / Role of Promoters and Micro RNA Backbone for Efficient Gene Silencing. Advanced Delivery and Therapeutic Applications of RNAi. John Wiley and Sons, 2013. pp. 83-108
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