Role of pleural mesothelial cells in the production of the submesothelial connective tissue matrix of lung

Stephen Israel Rennard, M. C. Jaurand, J. Bignon

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The pleura is comprised of a single layer of mesothelial cells resting on a complex layer of connective tissue. The ability of mesothelial cells to produce the components of this connective tissue was investigated using cultured rat mesothelial cells. These cells produced several components of extracellular matrix, including 6.8 ± 0.2 x 105 collagen pro-α-chains per cell per hour, which represented 3.09 ± 0.05% of all proteins synthesized by these cells. Chemical and immunologic criteria were used to demonstrate that these collagen chains included those of collagen types I, III, and IV. In addition, these cells produced elastin, as well as the connective tissue glycoproteins laminin and fibronectin. Moreover, electron microscopic studies revealed that lung mesothelial cells were capable of organizing these components into complex structures that resembled components of the extracellular matrix (thick collagen fibers, the amorphous component of elastic fibers, and basement membranelike structures), and restricted the formation of these structures to the basal region below the cells in culture. Thus, pleural mesothelial cells are active sources of a variety of connective tissue macromolecules found beneath mesothelial cells in situ, and can assemble these components into structures resembling the pleural extracellular matrix.

Original languageEnglish (US)
Pages (from-to)267-274
Number of pages8
JournalAmerican Review of Respiratory Disease
Volume130
Issue number2
StatePublished - Jan 1 1984

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Connective Tissue
Lung
Extracellular Matrix
Collagen
Collagen Type III
Elastic Tissue
Collagen Type IV
Pleura
Elastin
Laminin
Collagen Type I
Fibronectins
Glycoproteins
Cell Culture Techniques
Electrons

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Role of pleural mesothelial cells in the production of the submesothelial connective tissue matrix of lung. / Rennard, Stephen Israel; Jaurand, M. C.; Bignon, J.

In: American Review of Respiratory Disease, Vol. 130, No. 2, 01.01.1984, p. 267-274.

Research output: Contribution to journalArticle

Rennard, Stephen Israel ; Jaurand, M. C. ; Bignon, J. / Role of pleural mesothelial cells in the production of the submesothelial connective tissue matrix of lung. In: American Review of Respiratory Disease. 1984 ; Vol. 130, No. 2. pp. 267-274.
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