Role of neutrophils in endotoxin-mediated microvascular injury in hamsters

T. Matsuda, I. Rubinstein, R. A. Robbins, S. Koyama, W. L. Joyner, Stephen Israel Rennard

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The purpose of this study was to examine the role of circulating neutrophils in endotoxin-induced increase in microvascular permeability in vivo. Fifteen hamsters were anesthetized, and a plastic chamber was placed in each cheek pouch to observe the microvasculature. Fluorescein-labeled dextran (FITC-D, 150 kDa) was injected intravenously, and changes in leaky sites and FITC-D clearance were measured in three groups: control (saline, n = 4), endotoxin suffusion (n = 6), and endotoxin suffusion after neutropenia induction (n = 5). We found a significant increase in leaky sites and FITC-D clearance with endotoxin (45 ± 18/cm2 and 20 ± 6 x 10-6 ml/min, respectively; mean ± SD, P < 0.05) in comparison to control (7 ± 6/cm2 and 7 ± 5 X 10-6 ml/min) and endotoxin suffusion in neutropenic animals (19 ± 11/cm2 and 12 ± 4 x 10-6 ml/min). There was a significant correlation between the number of leaky sites and FITC-D clearance (r = 0.91, P < 0.01) and between the number of circulating neutrophils and FITC-D clearance (r = 0.87, P < 0.01). We conclude that endotoxin-mediated increase in microvascular permeability in the peripheral circulation is dependent in part on circulating neutrophils.

Original languageEnglish (US)
Pages (from-to)307-313
Number of pages7
JournalJournal of Applied Physiology
Volume71
Issue number1
StatePublished - Jan 1 1991

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Endotoxins
Fluorescein-5-isothiocyanate
Cricetinae
Neutrophils
Wounds and Injuries
Capillary Permeability
Cheek
Microvessels
Neutropenia
Plastics
Control Groups

Keywords

  • Escherichia coli
  • blood vessels
  • mechlorethamine hydrochloride
  • microvascular clearance
  • sepsis

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Matsuda, T., Rubinstein, I., Robbins, R. A., Koyama, S., Joyner, W. L., & Rennard, S. I. (1991). Role of neutrophils in endotoxin-mediated microvascular injury in hamsters. Journal of Applied Physiology, 71(1), 307-313.

Role of neutrophils in endotoxin-mediated microvascular injury in hamsters. / Matsuda, T.; Rubinstein, I.; Robbins, R. A.; Koyama, S.; Joyner, W. L.; Rennard, Stephen Israel.

In: Journal of Applied Physiology, Vol. 71, No. 1, 01.01.1991, p. 307-313.

Research output: Contribution to journalArticle

Matsuda, T, Rubinstein, I, Robbins, RA, Koyama, S, Joyner, WL & Rennard, SI 1991, 'Role of neutrophils in endotoxin-mediated microvascular injury in hamsters', Journal of Applied Physiology, vol. 71, no. 1, pp. 307-313.
Matsuda T, Rubinstein I, Robbins RA, Koyama S, Joyner WL, Rennard SI. Role of neutrophils in endotoxin-mediated microvascular injury in hamsters. Journal of Applied Physiology. 1991 Jan 1;71(1):307-313.
Matsuda, T. ; Rubinstein, I. ; Robbins, R. A. ; Koyama, S. ; Joyner, W. L. ; Rennard, Stephen Israel. / Role of neutrophils in endotoxin-mediated microvascular injury in hamsters. In: Journal of Applied Physiology. 1991 ; Vol. 71, No. 1. pp. 307-313.
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