Role of keratan sulfate expression in human pancreatic cancer malignancy

Premila D. Leiphrakpam, Prathamesh P. Patil, Neeley Remmers, Benjamin Swanson, Paul M. Grandgenett, Fang Qiu, Fang Yu, Prakash Radhakrishnan

Research output: Contribution to journalArticle

Abstract

Keratan sulfate (KS) is a sulfated linear polymer of N-acetyllactosamine. Proteoglycans carrying keratan sulfate epitopes were majorly observed in cornea, cartilage and brain; and mainly involved in embryonic development, cornea transparency, and wound healing process. Recently, expression of KS in cancer has been shown to be highly associated with advanced tumor grade and poor prognosis. Therefore, we aimed to identify the expression of KS epitope in human pancreatic cancer primary and metastatic tumor lesions. Immunohistochemical analysis of KS expression was performed on primary pancreatic tumors and metastatic tissues. We observed an increased expression of KS epitope on primary tumor tissues compared to uninvolved normal and tumor stroma; and is associated with worse overall survival. Moreover, lung metastatic tumors show a higher-level expression of KS compared to primary tumors. Interestingly, KS biosynthesis specific glycosyltransferases expression was differentially regulated in metastatic pancreatic tumors. Taken together, these results indicate that aberrant expression of KS is predictive of pancreatic cancer progression and metastasis and may serve as a novel prognostic biomarker for pancreatic cancer.

Original languageEnglish (US)
Article number9665
JournalScientific reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Keratan Sulfate
Pancreatic Neoplasms
Neoplasms
Epitopes
Cornea
Glycosyltransferases
Proteoglycans
Wound Healing
Cartilage
Embryonic Development
Polymers
Biomarkers
Neoplasm Metastasis
Lung

ASJC Scopus subject areas

  • General

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Role of keratan sulfate expression in human pancreatic cancer malignancy. / Leiphrakpam, Premila D.; Patil, Prathamesh P.; Remmers, Neeley; Swanson, Benjamin; Grandgenett, Paul M.; Qiu, Fang; Yu, Fang; Radhakrishnan, Prakash.

In: Scientific reports, Vol. 9, No. 1, 9665, 01.12.2019.

Research output: Contribution to journalArticle

Leiphrakpam, Premila D. ; Patil, Prathamesh P. ; Remmers, Neeley ; Swanson, Benjamin ; Grandgenett, Paul M. ; Qiu, Fang ; Yu, Fang ; Radhakrishnan, Prakash. / Role of keratan sulfate expression in human pancreatic cancer malignancy. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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abstract = "Keratan sulfate (KS) is a sulfated linear polymer of N-acetyllactosamine. Proteoglycans carrying keratan sulfate epitopes were majorly observed in cornea, cartilage and brain; and mainly involved in embryonic development, cornea transparency, and wound healing process. Recently, expression of KS in cancer has been shown to be highly associated with advanced tumor grade and poor prognosis. Therefore, we aimed to identify the expression of KS epitope in human pancreatic cancer primary and metastatic tumor lesions. Immunohistochemical analysis of KS expression was performed on primary pancreatic tumors and metastatic tissues. We observed an increased expression of KS epitope on primary tumor tissues compared to uninvolved normal and tumor stroma; and is associated with worse overall survival. Moreover, lung metastatic tumors show a higher-level expression of KS compared to primary tumors. Interestingly, KS biosynthesis specific glycosyltransferases expression was differentially regulated in metastatic pancreatic tumors. Taken together, these results indicate that aberrant expression of KS is predictive of pancreatic cancer progression and metastasis and may serve as a novel prognostic biomarker for pancreatic cancer.",
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AU - Remmers, Neeley

AU - Swanson, Benjamin

AU - Grandgenett, Paul M.

AU - Qiu, Fang

AU - Yu, Fang

AU - Radhakrishnan, Prakash

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