Role of extracellular iron in the action of the quinone antibiotic streptonigrin: Mechanisms of killing and resistance of Neisseria gonorrhoeae

M. S. Cohen, Y. Chai, B. E. Britigan, W. McKenna, J. Adams, T. Svendsen, K. Bean, D. J. Hassett, P. F. Sparling

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The quinone antibiotic streptonigrin is believed to kill bacteria by promoting formation of oxygen radicals. This antibiotic has also been used to select resistant bacterial mutants, some of which vary in iron utilization. We examined the effects of streptonigrin on Neisseria gonorrhoeae and several types of gonococcal mutants. Streptonigrin (0.025 μg/ml) efficiently killed gonococcal strain FA1090, and this effect depended on iron. Streptonigrin-resistant mutant FA6271 had normal iron uptake but was moderately deficient in total iron. Resistance most likely resulted from failure of FA6271 to divert electrons to streptonigrin, as demonstrated by a reduction in KCN-insensitive respiration (a hallmark of the action of quinones) and superoxide formation. Other mutants selected for inability to use human iron-binding proteins (strains FA6273 and FA6275) had no increase in streptonigrin MIC and no decrease in KCN-insensitive respiration. Mutants did not demonstrate an increase in superoxide dismutase or catalase. Streptonigrin killing of gonococci depended on a reaction(s) in which extracellular iron was important, presumably because iron was required for catalysis of hydroxyl radical. The results suggest that a membrane component may be a target for the actions of streptonigrin.

Original languageEnglish (US)
Pages (from-to)1507-1513
Number of pages7
JournalAntimicrobial Agents and Chemotherapy
Volume31
Issue number10
DOIs
StatePublished - Jan 1 1987

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Streptonigrin
Neisseria gonorrhoeae
Iron
Anti-Bacterial Agents
Respiration
Iron-Binding Proteins
Quinones
benzoquinone
Catalysis
Superoxides
Hydroxyl Radical
Catalase
Superoxide Dismutase
Reactive Oxygen Species
Electrons
Bacteria

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Role of extracellular iron in the action of the quinone antibiotic streptonigrin : Mechanisms of killing and resistance of Neisseria gonorrhoeae. / Cohen, M. S.; Chai, Y.; Britigan, B. E.; McKenna, W.; Adams, J.; Svendsen, T.; Bean, K.; Hassett, D. J.; Sparling, P. F.

In: Antimicrobial Agents and Chemotherapy, Vol. 31, No. 10, 01.01.1987, p. 1507-1513.

Research output: Contribution to journalArticle

Cohen, M. S. ; Chai, Y. ; Britigan, B. E. ; McKenna, W. ; Adams, J. ; Svendsen, T. ; Bean, K. ; Hassett, D. J. ; Sparling, P. F. / Role of extracellular iron in the action of the quinone antibiotic streptonigrin : Mechanisms of killing and resistance of Neisseria gonorrhoeae. In: Antimicrobial Agents and Chemotherapy. 1987 ; Vol. 31, No. 10. pp. 1507-1513.
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