Role of adenosine A2B receptor signaling in contribution of cardiac mesenchymal stem-like cells to myocardial scar formation

Sergey Ryzhov, Bong Hwan Sung, Qinkun Zhang, Alissa Weaver, Richard J. Gumina, Italo Biaggioni, Igor Feoktistov

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Adenosine levels increase in ischemic hearts and contribute to the modulation of that pathological environment. We previously showed that A2B adenosine receptors on mouse cardiac Sca1+CD31 mesenchymal stromal cells upregulate secretion of paracrine factors that may contribute to the improvement in cardiac recovery seen when these cells are transplanted in infarcted hearts. In this study, we tested the hypothesis that A2B receptor signaling regulates the transition of Sca1+CD31 cells, which occurs after myocardial injury, into a myofibroblast phenotype that promotes myocardial repair and remodeling. In vitro, TGFβ1 induced the expression of the myofibroblast marker α-smooth muscle actin (αSMA) and increased collagen I generation in Sca1+CD31 cells. Stimulation of A2B receptors attenuated TGFβ1-induced collagen I secretion but had no effect on αSMA expression. In vivo, myocardial infarction resulted in a rapid increase in the numbers of αSMA-positive cardiac stromal cells by day 5 followed by a gradual decline. Genetic deletion of A2B receptors had no effect on the initial accumulation of αSMA-expressing stromal cells but hastened their subsequent decline; the numbers of αSMA-positive cells including Sca1+CD31 cells remained significantly higher in wild type compared with A2B knockout hearts. Thus, our study revealed a significant contribution of cardiac Sca1+CD31 cells to the accumulation of αSMA-expressing cells after infarction and implicated A2B receptor signaling in regulation of myocardial repair and remodeling by delaying deactivation of these cells. It is plausible that this phenomenon may contribute to the beneficial effects of transplantation of these cells to the injured heart.

Original languageEnglish (US)
Pages (from-to)477-486
Number of pages10
JournalPurinergic Signalling
Volume10
Issue number3
DOIs
StatePublished - Jan 1 2014

Fingerprint

Adenosine A2B Receptors
Mesenchymal Stromal Cells
Cicatrix
Smooth Muscle
Actins
Myofibroblasts
Stromal Cells
Collagen
Cell Transplantation
Adenosine
Infarction
Up-Regulation
Myocardial Infarction
Phenotype

Keywords

  • Adenosine
  • Adenosine A
  • Alpha-smooth muscle actin
  • Mesenchymal stromal cells
  • Myocardial infarction
  • Myofibroblasts
  • Receptor

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Role of adenosine A2B receptor signaling in contribution of cardiac mesenchymal stem-like cells to myocardial scar formation. / Ryzhov, Sergey; Sung, Bong Hwan; Zhang, Qinkun; Weaver, Alissa; Gumina, Richard J.; Biaggioni, Italo; Feoktistov, Igor.

In: Purinergic Signalling, Vol. 10, No. 3, 01.01.2014, p. 477-486.

Research output: Contribution to journalArticle

Ryzhov, S, Sung, BH, Zhang, Q, Weaver, A, Gumina, RJ, Biaggioni, I & Feoktistov, I 2014, 'Role of adenosine A2B receptor signaling in contribution of cardiac mesenchymal stem-like cells to myocardial scar formation', Purinergic Signalling, vol. 10, no. 3, pp. 477-486. https://doi.org/10.1007/s11302-014-9410-y
Ryzhov, Sergey ; Sung, Bong Hwan ; Zhang, Qinkun ; Weaver, Alissa ; Gumina, Richard J. ; Biaggioni, Italo ; Feoktistov, Igor. / Role of adenosine A2B receptor signaling in contribution of cardiac mesenchymal stem-like cells to myocardial scar formation. In: Purinergic Signalling. 2014 ; Vol. 10, No. 3. pp. 477-486.
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