Abstract
During cardiac c-looping, the heart transforms from a straight tube into a c-shaped tube, presenting the first evidence of left-right asymmetry in the embryo. C-looping consists of two primary deformation components: ventral bending and dextral rotation. This study examines the role of actin polymerization in bending of the heart tube. Exposure of stage 9-11 chick embryos to low concentrations of the actin polymerization inhibitors cytochalasin D (5 nM-2.0 μM) and latrunculin A (LA; 25 nM-2.0 μM) suppressed looping in a stage- and concentration-dependent manner in both whole embryos and isolated hearts. Local exposure of either the dorsal or ventral sides of isolated hearts to LA also inhibited looping, but less than global exposure, indicating that both sides contribute to the bending mechanism. Taken together, these data suggest that ongoing actin polymerization is required for the bending component of cardiac c-looping, and we speculate that polymerization-driven myocardial cell shape changes cause this deformation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1272-1286 |
| Number of pages | 15 |
| Journal | Developmental Dynamics |
| Volume | 233 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 1 2005 |
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Keywords
- Actin polymerization
- Cardiac looping
- Chick embryo
- Cytochalasin D
- Heart development
- Latrunculin A
ASJC Scopus subject areas
- Developmental Biology
Cite this
Role of actin polymerization in bending of the early heart tube. / Latacha, Kimberly S; Rémond, Mathieu C.; Ramasubramanian, Ashok; Chen, Amy Y.; Elson, Elliot L.; Taber, Larry A.
In: Developmental Dynamics, Vol. 233, No. 4, 01.08.2005, p. 1272-1286.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of actin polymerization in bending of the early heart tube
AU - Latacha, Kimberly S
AU - Rémond, Mathieu C.
AU - Ramasubramanian, Ashok
AU - Chen, Amy Y.
AU - Elson, Elliot L.
AU - Taber, Larry A.
PY - 2005/8/1
Y1 - 2005/8/1
N2 - During cardiac c-looping, the heart transforms from a straight tube into a c-shaped tube, presenting the first evidence of left-right asymmetry in the embryo. C-looping consists of two primary deformation components: ventral bending and dextral rotation. This study examines the role of actin polymerization in bending of the heart tube. Exposure of stage 9-11 chick embryos to low concentrations of the actin polymerization inhibitors cytochalasin D (5 nM-2.0 μM) and latrunculin A (LA; 25 nM-2.0 μM) suppressed looping in a stage- and concentration-dependent manner in both whole embryos and isolated hearts. Local exposure of either the dorsal or ventral sides of isolated hearts to LA also inhibited looping, but less than global exposure, indicating that both sides contribute to the bending mechanism. Taken together, these data suggest that ongoing actin polymerization is required for the bending component of cardiac c-looping, and we speculate that polymerization-driven myocardial cell shape changes cause this deformation.
AB - During cardiac c-looping, the heart transforms from a straight tube into a c-shaped tube, presenting the first evidence of left-right asymmetry in the embryo. C-looping consists of two primary deformation components: ventral bending and dextral rotation. This study examines the role of actin polymerization in bending of the heart tube. Exposure of stage 9-11 chick embryos to low concentrations of the actin polymerization inhibitors cytochalasin D (5 nM-2.0 μM) and latrunculin A (LA; 25 nM-2.0 μM) suppressed looping in a stage- and concentration-dependent manner in both whole embryos and isolated hearts. Local exposure of either the dorsal or ventral sides of isolated hearts to LA also inhibited looping, but less than global exposure, indicating that both sides contribute to the bending mechanism. Taken together, these data suggest that ongoing actin polymerization is required for the bending component of cardiac c-looping, and we speculate that polymerization-driven myocardial cell shape changes cause this deformation.
KW - Actin polymerization
KW - Cardiac looping
KW - Chick embryo
KW - Cytochalasin D
KW - Heart development
KW - Latrunculin A
UR - http://www.scopus.com/inward/record.url?scp=22244459129&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=22244459129&partnerID=8YFLogxK
U2 - 10.1002/dvdy.20488
DO - 10.1002/dvdy.20488
M3 - Article
VL - 233
SP - 1272
EP - 1286
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 4
ER -