Role of actin polymerization in bending of the early heart tube

Kimberly S. Latacha, Mathieu C. Rémond, Ashok Ramasubramanian, Amy Y. Chen, Elliot L. Elson, Larry A. Taber

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

During cardiac c-looping, the heart transforms from a straight tube into a c-shaped tube, presenting the first evidence of left-right asymmetry in the embryo. C-looping consists of two primary deformation components: ventral bending and dextral rotation. This study examines the role of actin polymerization in bending of the heart tube. Exposure of stage 9-11 chick embryos to low concentrations of the actin polymerization inhibitors cytochalasin D (5 nM-2.0 μM) and latrunculin A (LA; 25 nM-2.0 μM) suppressed looping in a stage- and concentration-dependent manner in both whole embryos and isolated hearts. Local exposure of either the dorsal or ventral sides of isolated hearts to LA also inhibited looping, but less than global exposure, indicating that both sides contribute to the bending mechanism. Taken together, these data suggest that ongoing actin polymerization is required for the bending component of cardiac c-looping, and we speculate that polymerization-driven myocardial cell shape changes cause this deformation.

Original languageEnglish (US)
Pages (from-to)1272-1286
Number of pages15
JournalDevelopmental Dynamics
Volume233
Issue number4
DOIs
StatePublished - Aug 1 2005

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Polymerization
Actins
Embryonic Structures
Cytochalasin D
Cell Shape
Chick Embryo

Keywords

  • Actin polymerization
  • Cardiac looping
  • Chick embryo
  • Cytochalasin D
  • Heart development
  • Latrunculin A

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Latacha, K. S., Rémond, M. C., Ramasubramanian, A., Chen, A. Y., Elson, E. L., & Taber, L. A. (2005). Role of actin polymerization in bending of the early heart tube. Developmental Dynamics, 233(4), 1272-1286. https://doi.org/10.1002/dvdy.20488

Role of actin polymerization in bending of the early heart tube. / Latacha, Kimberly S.; Rémond, Mathieu C.; Ramasubramanian, Ashok; Chen, Amy Y.; Elson, Elliot L.; Taber, Larry A.

In: Developmental Dynamics, Vol. 233, No. 4, 01.08.2005, p. 1272-1286.

Research output: Contribution to journalArticle

Latacha, KS, Rémond, MC, Ramasubramanian, A, Chen, AY, Elson, EL & Taber, LA 2005, 'Role of actin polymerization in bending of the early heart tube', Developmental Dynamics, vol. 233, no. 4, pp. 1272-1286. https://doi.org/10.1002/dvdy.20488
Latacha KS, Rémond MC, Ramasubramanian A, Chen AY, Elson EL, Taber LA. Role of actin polymerization in bending of the early heart tube. Developmental Dynamics. 2005 Aug 1;233(4):1272-1286. https://doi.org/10.1002/dvdy.20488
Latacha, Kimberly S. ; Rémond, Mathieu C. ; Ramasubramanian, Ashok ; Chen, Amy Y. ; Elson, Elliot L. ; Taber, Larry A. / Role of actin polymerization in bending of the early heart tube. In: Developmental Dynamics. 2005 ; Vol. 233, No. 4. pp. 1272-1286.
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