Role of α5β1 integrin in determining malignant properties of colon carcinoma cells

Jiangen Gong, Dan Hui Wang, Lu Zhe Sun, Elizabeth Zborowska, James K.V. Willson, Michael G Brattain

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

We characterized the expression of α5β1 integrin in two distinct phenotypes of colon carcinoma cell lines. Highly invasive colon cell lines (designated Group I cell lines) expressed higher levels of integrin α5β1 mRNA and protein than did poorly invasive colon cell lines (designated Group III cell lines). The relatively high expression of integrin α5β1 in Group I cell lines resulted in strong enhancement of cell adhesion to fibronectin (FN) tissue culture plates, whereas Group III cell lines showed little or no enhancement of cell adhesion by coating. There was no significant difference between Group I and Group III cell lines with respect to cell adhesion to laminin and collagen IV. Cell adhesion to FN in Group I cells was mainly mediated by integrin α5β1 because a monoclonal antiα5 subunit antibody could block cell adhesion to FN, whereas anti-α2 and anti-α3 antibodies had no effect on cell adhesion to FN. The divergence of α5β1 expression in these two distinct colon carcinoma phenotypes suggested that high expression of α5β1 might contribute to malignant progression in this model system. To test this hypothesis, GEO cells, a Group III cell line that did not express α5 integrin, were transfected with the α5 subunit. Stable transfection of α5 sense cDNA into a typical GEO-limiting dilution clone led to the expression of α5 subunit mRNA and cell surface α5β1 protein. The α5 sense transfectants showed enhanced attachment to FN-coated plates and were more tumorigenic when the cells were injected into athymicnude mice. These results indicate that inappropriately high α5β1 integrin expression contributes to malignant progression in colon carcinoma.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalCell Growth and Differentiation
Volume8
Issue number1
StatePublished - Jan 22 1997

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Integrins
Colon
Carcinoma
Cell Adhesion
Cell Line
Fibronectins
Phenotype
Messenger RNA
Laminin
Transfection
Anti-Idiotypic Antibodies
Membrane Proteins
Collagen
Complementary DNA
Clone Cells
Antibodies

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Gong, J., Wang, D. H., Sun, L. Z., Zborowska, E., Willson, J. K. V., & Brattain, M. G. (1997). Role of α5β1 integrin in determining malignant properties of colon carcinoma cells. Cell Growth and Differentiation, 8(1), 83-90.

Role of α5β1 integrin in determining malignant properties of colon carcinoma cells. / Gong, Jiangen; Wang, Dan Hui; Sun, Lu Zhe; Zborowska, Elizabeth; Willson, James K.V.; Brattain, Michael G.

In: Cell Growth and Differentiation, Vol. 8, No. 1, 22.01.1997, p. 83-90.

Research output: Contribution to journalArticle

Gong, J, Wang, DH, Sun, LZ, Zborowska, E, Willson, JKV & Brattain, MG 1997, 'Role of α5β1 integrin in determining malignant properties of colon carcinoma cells', Cell Growth and Differentiation, vol. 8, no. 1, pp. 83-90.
Gong J, Wang DH, Sun LZ, Zborowska E, Willson JKV, Brattain MG. Role of α5β1 integrin in determining malignant properties of colon carcinoma cells. Cell Growth and Differentiation. 1997 Jan 22;8(1):83-90.
Gong, Jiangen ; Wang, Dan Hui ; Sun, Lu Zhe ; Zborowska, Elizabeth ; Willson, James K.V. ; Brattain, Michael G. / Role of α5β1 integrin in determining malignant properties of colon carcinoma cells. In: Cell Growth and Differentiation. 1997 ; Vol. 8, No. 1. pp. 83-90.
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