RNAi screening reveals requirement for host cell secretory pathway in infection by diverse families of negative-strand RNA viruses

Debasis Panda, Anshuman Das, Phat X. Dinh, Sakthivel Subramaniam, Debasis Nayak, Nicholas J. Barrows, James L. Pearson, Jesse Thompson, David Lee Kelly, Istvan Ladunga, Asit K Pattnaik

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Negative-strand (NS) RNA viruses comprise many pathogens that cause serious diseases in humans and animals. Despite their clinical importance, little is known about the host factors required for their infection. Using vesicular stomatitis virus (VSV), a prototypic NS RNA virus in the family Rhabdoviridae, we conducted a human genomewide siRNA screen and identified 72 host genes required for viral infection. Many of these identified genes were also required for infection by two other NS RNA viruses, the lymphocytic choriomeningitis virus of the Arenaviridae family and human parainfluenza virus type 3 of the Paramyxoviridae family. Genes affecting different stages of VSV infection, such as entry/uncoating, gene expression, and assembly/release,were identified.Depletion of the proteins of the coatomer complex I or its upstream effectors ARF1 or GBF1 led to detection of reduced levels of VSV RNA. Coatomer complex I was also required for infection of lymphocytic choriomeningitis virus and human parainfluenza virus type 3. These results highlight the evolutionarily conserved requirements for gene expression of diverse families of NS RNA viruses and demonstrate the involvement of host cell secretory pathway in the process.

Original languageEnglish (US)
Pages (from-to)19036-19041
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number47
DOIs
StatePublished - Nov 22 2011

Fingerprint

Human parainfluenza virus 3
Secretory Pathway
RNA Viruses
RNA Interference
Vesicular Stomatitis
Lymphocytic choriomeningitis virus
Infection
Virus Diseases
Arenaviridae
Coatomer Protein
Paramyxoviridae
Rhabdoviridae
Genes
Gene Expression
Small Interfering RNA
Viruses

Keywords

  • Host cell factors for virus infection
  • RNA interference
  • Transcription and replication

ASJC Scopus subject areas

  • General

Cite this

RNAi screening reveals requirement for host cell secretory pathway in infection by diverse families of negative-strand RNA viruses. / Panda, Debasis; Das, Anshuman; Dinh, Phat X.; Subramaniam, Sakthivel; Nayak, Debasis; Barrows, Nicholas J.; Pearson, James L.; Thompson, Jesse; Kelly, David Lee; Ladunga, Istvan; Pattnaik, Asit K.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 47, 22.11.2011, p. 19036-19041.

Research output: Contribution to journalArticle

Panda, Debasis ; Das, Anshuman ; Dinh, Phat X. ; Subramaniam, Sakthivel ; Nayak, Debasis ; Barrows, Nicholas J. ; Pearson, James L. ; Thompson, Jesse ; Kelly, David Lee ; Ladunga, Istvan ; Pattnaik, Asit K. / RNAi screening reveals requirement for host cell secretory pathway in infection by diverse families of negative-strand RNA viruses. In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 47. pp. 19036-19041.
@article{5823606bd8bc45ba9d26aaf1b8a6865c,
title = "RNAi screening reveals requirement for host cell secretory pathway in infection by diverse families of negative-strand RNA viruses",
abstract = "Negative-strand (NS) RNA viruses comprise many pathogens that cause serious diseases in humans and animals. Despite their clinical importance, little is known about the host factors required for their infection. Using vesicular stomatitis virus (VSV), a prototypic NS RNA virus in the family Rhabdoviridae, we conducted a human genomewide siRNA screen and identified 72 host genes required for viral infection. Many of these identified genes were also required for infection by two other NS RNA viruses, the lymphocytic choriomeningitis virus of the Arenaviridae family and human parainfluenza virus type 3 of the Paramyxoviridae family. Genes affecting different stages of VSV infection, such as entry/uncoating, gene expression, and assembly/release,were identified.Depletion of the proteins of the coatomer complex I or its upstream effectors ARF1 or GBF1 led to detection of reduced levels of VSV RNA. Coatomer complex I was also required for infection of lymphocytic choriomeningitis virus and human parainfluenza virus type 3. These results highlight the evolutionarily conserved requirements for gene expression of diverse families of NS RNA viruses and demonstrate the involvement of host cell secretory pathway in the process.",
keywords = "Host cell factors for virus infection, RNA interference, Transcription and replication",
author = "Debasis Panda and Anshuman Das and Dinh, {Phat X.} and Sakthivel Subramaniam and Debasis Nayak and Barrows, {Nicholas J.} and Pearson, {James L.} and Jesse Thompson and Kelly, {David Lee} and Istvan Ladunga and Pattnaik, {Asit K}",
year = "2011",
month = "11",
day = "22",
doi = "10.1073/pnas.1113643108",
language = "English (US)",
volume = "108",
pages = "19036--19041",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "47",

}

TY - JOUR

T1 - RNAi screening reveals requirement for host cell secretory pathway in infection by diverse families of negative-strand RNA viruses

AU - Panda, Debasis

AU - Das, Anshuman

AU - Dinh, Phat X.

AU - Subramaniam, Sakthivel

AU - Nayak, Debasis

AU - Barrows, Nicholas J.

AU - Pearson, James L.

AU - Thompson, Jesse

AU - Kelly, David Lee

AU - Ladunga, Istvan

AU - Pattnaik, Asit K

PY - 2011/11/22

Y1 - 2011/11/22

N2 - Negative-strand (NS) RNA viruses comprise many pathogens that cause serious diseases in humans and animals. Despite their clinical importance, little is known about the host factors required for their infection. Using vesicular stomatitis virus (VSV), a prototypic NS RNA virus in the family Rhabdoviridae, we conducted a human genomewide siRNA screen and identified 72 host genes required for viral infection. Many of these identified genes were also required for infection by two other NS RNA viruses, the lymphocytic choriomeningitis virus of the Arenaviridae family and human parainfluenza virus type 3 of the Paramyxoviridae family. Genes affecting different stages of VSV infection, such as entry/uncoating, gene expression, and assembly/release,were identified.Depletion of the proteins of the coatomer complex I or its upstream effectors ARF1 or GBF1 led to detection of reduced levels of VSV RNA. Coatomer complex I was also required for infection of lymphocytic choriomeningitis virus and human parainfluenza virus type 3. These results highlight the evolutionarily conserved requirements for gene expression of diverse families of NS RNA viruses and demonstrate the involvement of host cell secretory pathway in the process.

AB - Negative-strand (NS) RNA viruses comprise many pathogens that cause serious diseases in humans and animals. Despite their clinical importance, little is known about the host factors required for their infection. Using vesicular stomatitis virus (VSV), a prototypic NS RNA virus in the family Rhabdoviridae, we conducted a human genomewide siRNA screen and identified 72 host genes required for viral infection. Many of these identified genes were also required for infection by two other NS RNA viruses, the lymphocytic choriomeningitis virus of the Arenaviridae family and human parainfluenza virus type 3 of the Paramyxoviridae family. Genes affecting different stages of VSV infection, such as entry/uncoating, gene expression, and assembly/release,were identified.Depletion of the proteins of the coatomer complex I or its upstream effectors ARF1 or GBF1 led to detection of reduced levels of VSV RNA. Coatomer complex I was also required for infection of lymphocytic choriomeningitis virus and human parainfluenza virus type 3. These results highlight the evolutionarily conserved requirements for gene expression of diverse families of NS RNA viruses and demonstrate the involvement of host cell secretory pathway in the process.

KW - Host cell factors for virus infection

KW - RNA interference

KW - Transcription and replication

UR - http://www.scopus.com/inward/record.url?scp=82755168802&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82755168802&partnerID=8YFLogxK

U2 - 10.1073/pnas.1113643108

DO - 10.1073/pnas.1113643108

M3 - Article

C2 - 22065774

AN - SCOPUS:82755168802

VL - 108

SP - 19036

EP - 19041

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 47

ER -