RNA polymerase activity associated with bacteriophage phi6

James L Van Etten, A. K. Vidaver, R. K. Koski, J. S. Semancik

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The Pseudomonas phaseolicola bacteriophage phi6 incorporated labeled UTP into an acid insoluble precipitate. Incorporation was dependent on the presence of manganese acetate, ATP, GTP, CTP, and a short heat treatment of the phage; the reaction was stimulated by NH4Cl. The substitution of 14C ATP, -CTP or -GTP for UTP, together with the appropriate unlabeled ribonucleoside triphosphates, disclosed that CMP was incorporated to the greatest extent followed by GMP, UMP, and AMP. Radioactive RNAs formed by the reaction were resistant to RNases A and T1 in high salt but susceptible to these nucleases in low salt. The labeled RNA cosedimented and coelectrophoresed with phi6 double stranded (ds) RNA. However, the distribution of the radioactivity into the three ds RNA components varied depending on the 14C ribonucleoside triphosphate used in the reaction. The incorporation of UMP was primarily into the 2 smaller ds RNA segments, GMP primarily into the large ds RNA segment, and CMP and AMP were about equally distributed into all 3 ds RNA segments.

Original languageEnglish (US)
Pages (from-to)464-471
Number of pages8
JournalJournal of virology
Volume12
Issue number3
StatePublished - Dec 1 1973

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Double-Stranded RNA
double-stranded RNA
DNA-Directed RNA Polymerases
DNA-directed RNA polymerase
bacteriophages
Bacteriophages
ribonucleosides
Uridine Monophosphate
Cytidine Monophosphate
Ribonucleosides
Cytidine Triphosphate
Uridine Triphosphate
Adenosine Monophosphate
Guanosine Triphosphate
Pseudomonas Phages
Salts
Adenosine Triphosphate
RNA
Ribonuclease T1
salts

ASJC Scopus subject areas

  • Immunology

Cite this

Van Etten, J. L., Vidaver, A. K., Koski, R. K., & Semancik, J. S. (1973). RNA polymerase activity associated with bacteriophage phi6. Journal of virology, 12(3), 464-471.

RNA polymerase activity associated with bacteriophage phi6. / Van Etten, James L; Vidaver, A. K.; Koski, R. K.; Semancik, J. S.

In: Journal of virology, Vol. 12, No. 3, 01.12.1973, p. 464-471.

Research output: Contribution to journalArticle

Van Etten, JL, Vidaver, AK, Koski, RK & Semancik, JS 1973, 'RNA polymerase activity associated with bacteriophage phi6', Journal of virology, vol. 12, no. 3, pp. 464-471.
Van Etten JL, Vidaver AK, Koski RK, Semancik JS. RNA polymerase activity associated with bacteriophage phi6. Journal of virology. 1973 Dec 1;12(3):464-471.
Van Etten, James L ; Vidaver, A. K. ; Koski, R. K. ; Semancik, J. S. / RNA polymerase activity associated with bacteriophage phi6. In: Journal of virology. 1973 ; Vol. 12, No. 3. pp. 464-471.
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