Richter's syndrome with different immunoglobulin light chain types: Molecular and cytogenetic features indicate a common clonal origin

H. Nakamine, A. S. Masih, W. G. Sanger, R. S. Wickert, D. W. Mitchell, James Olen Armitage, D. D. Weisenburger

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

To investigate the issue of clonality in Richter's syndrome, phenotypic, molecular genetic, and cytogenetic studies were performed on tumor tissue from a patient with concurrent chronic lymphocytic leukemia and diffuse large cell lymphoma in a single lymph node specimen. The tumor was biphenotypic for immunoglobulin (Ig) expression with surface Igλ-positive chronic lymphocytic leukemia and surface and cytoplasmic Igκ-positive diffuse large cell lymphoma. DNA samples prepared from areas of the lymph node rich in chronic lymphocytic leukemia cells and diffuse large cell lymphoma cells were examined in parallel. Identical Ig heavy chain gene rearrangements were detected in the BamHI and EcoRI digests of the two samples, but the patterns of rearrangement were different in the HindIII and PstI digests. Because it is very unlikely that multiple rearranged Ig heavy chain gene fragments of identical size would be found in more than one enzyme digest from two independently derived B-cell clones, it is probable that the two processes originated from a single clone. Modifications after rearrangement probably accounted for the differing band sizes seen in the HindIII and PstI digests. These conclusions are supported by cytogenetic analysis, which revealed two clones with a common primary abnormality (trisomy 12), one of which also exhibited secondary abnormalities. Therefore, Richter's syndrome may represent a composite tumor of common clonal origin, even when differences in light chain expression are identified.

Original languageEnglish (US)
Pages (from-to)656-663
Number of pages8
JournalAmerican journal of clinical pathology
Volume97
Issue number5
DOIs
StatePublished - Jan 1 1992

Fingerprint

Immunoglobulin Light Chains
Lymphoma, Large B-Cell, Diffuse
B-Cell Chronic Lymphocytic Leukemia
Cytogenetics
Immunoglobulin Heavy Chain Genes
B-Cell Antigen Receptors
Clone Cells
Lymph Nodes
Neoplasms
Gene Rearrangement
Cytogenetic Analysis
Trisomy
Immunoglobulins
Molecular Biology
B-Lymphocytes
Light
DNA
Enzymes

Keywords

  • Biphenotypic lymphoma
  • Chronic lymphocytic leukemia
  • Clonal evolution
  • Clonal origin
  • Non-Hodgkin's lymphoma
  • Richter's syndrome

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Richter's syndrome with different immunoglobulin light chain types : Molecular and cytogenetic features indicate a common clonal origin. / Nakamine, H.; Masih, A. S.; Sanger, W. G.; Wickert, R. S.; Mitchell, D. W.; Armitage, James Olen; Weisenburger, D. D.

In: American journal of clinical pathology, Vol. 97, No. 5, 01.01.1992, p. 656-663.

Research output: Contribution to journalArticle

Nakamine, H. ; Masih, A. S. ; Sanger, W. G. ; Wickert, R. S. ; Mitchell, D. W. ; Armitage, James Olen ; Weisenburger, D. D. / Richter's syndrome with different immunoglobulin light chain types : Molecular and cytogenetic features indicate a common clonal origin. In: American journal of clinical pathology. 1992 ; Vol. 97, No. 5. pp. 656-663.
@article{e423be31724b40639e4a1de996035728,
title = "Richter's syndrome with different immunoglobulin light chain types: Molecular and cytogenetic features indicate a common clonal origin",
abstract = "To investigate the issue of clonality in Richter's syndrome, phenotypic, molecular genetic, and cytogenetic studies were performed on tumor tissue from a patient with concurrent chronic lymphocytic leukemia and diffuse large cell lymphoma in a single lymph node specimen. The tumor was biphenotypic for immunoglobulin (Ig) expression with surface Igλ-positive chronic lymphocytic leukemia and surface and cytoplasmic Igκ-positive diffuse large cell lymphoma. DNA samples prepared from areas of the lymph node rich in chronic lymphocytic leukemia cells and diffuse large cell lymphoma cells were examined in parallel. Identical Ig heavy chain gene rearrangements were detected in the BamHI and EcoRI digests of the two samples, but the patterns of rearrangement were different in the HindIII and PstI digests. Because it is very unlikely that multiple rearranged Ig heavy chain gene fragments of identical size would be found in more than one enzyme digest from two independently derived B-cell clones, it is probable that the two processes originated from a single clone. Modifications after rearrangement probably accounted for the differing band sizes seen in the HindIII and PstI digests. These conclusions are supported by cytogenetic analysis, which revealed two clones with a common primary abnormality (trisomy 12), one of which also exhibited secondary abnormalities. Therefore, Richter's syndrome may represent a composite tumor of common clonal origin, even when differences in light chain expression are identified.",
keywords = "Biphenotypic lymphoma, Chronic lymphocytic leukemia, Clonal evolution, Clonal origin, Non-Hodgkin's lymphoma, Richter's syndrome",
author = "H. Nakamine and Masih, {A. S.} and Sanger, {W. G.} and Wickert, {R. S.} and Mitchell, {D. W.} and Armitage, {James Olen} and Weisenburger, {D. D.}",
year = "1992",
month = "1",
day = "1",
doi = "10.1093/ajcp/97.5.656",
language = "English (US)",
volume = "97",
pages = "656--663",
journal = "American Journal of Clinical Pathology",
issn = "0002-9173",
publisher = "American Society of Clinical Pathologists",
number = "5",

}

TY - JOUR

T1 - Richter's syndrome with different immunoglobulin light chain types

T2 - Molecular and cytogenetic features indicate a common clonal origin

AU - Nakamine, H.

AU - Masih, A. S.

AU - Sanger, W. G.

AU - Wickert, R. S.

AU - Mitchell, D. W.

AU - Armitage, James Olen

AU - Weisenburger, D. D.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - To investigate the issue of clonality in Richter's syndrome, phenotypic, molecular genetic, and cytogenetic studies were performed on tumor tissue from a patient with concurrent chronic lymphocytic leukemia and diffuse large cell lymphoma in a single lymph node specimen. The tumor was biphenotypic for immunoglobulin (Ig) expression with surface Igλ-positive chronic lymphocytic leukemia and surface and cytoplasmic Igκ-positive diffuse large cell lymphoma. DNA samples prepared from areas of the lymph node rich in chronic lymphocytic leukemia cells and diffuse large cell lymphoma cells were examined in parallel. Identical Ig heavy chain gene rearrangements were detected in the BamHI and EcoRI digests of the two samples, but the patterns of rearrangement were different in the HindIII and PstI digests. Because it is very unlikely that multiple rearranged Ig heavy chain gene fragments of identical size would be found in more than one enzyme digest from two independently derived B-cell clones, it is probable that the two processes originated from a single clone. Modifications after rearrangement probably accounted for the differing band sizes seen in the HindIII and PstI digests. These conclusions are supported by cytogenetic analysis, which revealed two clones with a common primary abnormality (trisomy 12), one of which also exhibited secondary abnormalities. Therefore, Richter's syndrome may represent a composite tumor of common clonal origin, even when differences in light chain expression are identified.

AB - To investigate the issue of clonality in Richter's syndrome, phenotypic, molecular genetic, and cytogenetic studies were performed on tumor tissue from a patient with concurrent chronic lymphocytic leukemia and diffuse large cell lymphoma in a single lymph node specimen. The tumor was biphenotypic for immunoglobulin (Ig) expression with surface Igλ-positive chronic lymphocytic leukemia and surface and cytoplasmic Igκ-positive diffuse large cell lymphoma. DNA samples prepared from areas of the lymph node rich in chronic lymphocytic leukemia cells and diffuse large cell lymphoma cells were examined in parallel. Identical Ig heavy chain gene rearrangements were detected in the BamHI and EcoRI digests of the two samples, but the patterns of rearrangement were different in the HindIII and PstI digests. Because it is very unlikely that multiple rearranged Ig heavy chain gene fragments of identical size would be found in more than one enzyme digest from two independently derived B-cell clones, it is probable that the two processes originated from a single clone. Modifications after rearrangement probably accounted for the differing band sizes seen in the HindIII and PstI digests. These conclusions are supported by cytogenetic analysis, which revealed two clones with a common primary abnormality (trisomy 12), one of which also exhibited secondary abnormalities. Therefore, Richter's syndrome may represent a composite tumor of common clonal origin, even when differences in light chain expression are identified.

KW - Biphenotypic lymphoma

KW - Chronic lymphocytic leukemia

KW - Clonal evolution

KW - Clonal origin

KW - Non-Hodgkin's lymphoma

KW - Richter's syndrome

UR - http://www.scopus.com/inward/record.url?scp=0026651069&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026651069&partnerID=8YFLogxK

U2 - 10.1093/ajcp/97.5.656

DO - 10.1093/ajcp/97.5.656

M3 - Article

C2 - 1575210

AN - SCOPUS:0026651069

VL - 97

SP - 656

EP - 663

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

SN - 0002-9173

IS - 5

ER -