Rice fertilization-independent Endosperm1 regulates seed size under heat stress by controlling early endosperm development

Jing J. Folsom, Kevin Begcy, Xiaojuan Hao, Dong Wang, Harkamal Walia

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Although heat stress reduces seed size in rice (Oryza sativa), little is known about the molecular mechanisms underlying the observed reduction in seed size and yield. To elucidate the mechanistic basis of heat sensitivity and reduced seed size, we imposed a moderate (34°C) and a high (42°C) heat stress treatment on developing rice seeds during the postfertilization stage. Both stress treatments reduced the final seed size. At a cellular level, the moderate heat stress resulted in precocious endosperm cellularization, whereas severe heat-stressed seeds failed to cellularize. Initiation of endosperm cellularization is a critical developmental transition required for normal seed development, and it is controlled by Polycomb Repressive Complex2 (PRC2) in Arabidopsis (Arabidopsis thaliana). We observed that a member of PRC2 called Fertilization-Independent Endosperm1 (OsFIE1) was sensitive to temperature changes, and its expression was negatively correlated with the duration of the syncytial stage during heat stress. Seeds from plants overexpressing OsFIE1 had reduced seed size and exhibited precocious cellularization. The DNA methylation status and a repressive histone modification of OsFIE1 were observed to be temperature sensitive. Our data suggested that the thermal sensitivity of seed enlargement could partly be caused by altered epigenetic regulation of endosperm development during the transition from the syncytial to the cellularized state.

Original languageEnglish (US)
Pages (from-to)238-248
Number of pages11
JournalPlant Physiology
Volume165
Issue number1
DOIs
StatePublished - Jan 1 2014

Fingerprint

Endosperm
Fertilization
endosperm
heat stress
Seeds
Hot Temperature
rice
seeds
heat
Arabidopsis
Histone Code
Oryza
Temperature
DNA methylation
seed development
histones
epigenetics
DNA Methylation
Epigenomics
Oryza sativa

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Rice fertilization-independent Endosperm1 regulates seed size under heat stress by controlling early endosperm development. / Folsom, Jing J.; Begcy, Kevin; Hao, Xiaojuan; Wang, Dong; Walia, Harkamal.

In: Plant Physiology, Vol. 165, No. 1, 01.01.2014, p. 238-248.

Research output: Contribution to journalArticle

Folsom, Jing J. ; Begcy, Kevin ; Hao, Xiaojuan ; Wang, Dong ; Walia, Harkamal. / Rice fertilization-independent Endosperm1 regulates seed size under heat stress by controlling early endosperm development. In: Plant Physiology. 2014 ; Vol. 165, No. 1. pp. 238-248.
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