RhoA Kinase (Rock) and p90 Ribosomal S6 Kinase (p90Rsk) phosphorylation of the sodium hydrogen exchanger (NHE1) is required for lysophosphatidic acid-induced transport, cytoskeletal organization and migration

Mark A. Wallert, Daniel Hammes, Tony Nguyen, Lea Kiefer, Nick Berthelsen, Andrew Kern, Kristina Anderson-Tiege, John B. Shabb, Wallace W. Muhonen, Bryon D. Grove, Joseph J. Provost

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The sodium hydrogen exchanger isoform one (NHE1) plays a critical role coordinating asymmetric events at the leading edge of migrating cells and is regulated by a number of phosphorylation events influencing both the ion transport and cytoskeletal anchoring required for directed migration. Lysophosphatidic acid (LPA) activation of RhoA kinase (Rock) and the Ras-ERK growth factor pathway induces cytoskeletal reorganization, activates NHE1 and induces an increase in cell motility. We report that both Rock I and II stoichiometrically phosphorylate NHE1 at threonine 653 in vitro using mass spectrometry and reconstituted kinase assays. In fibroblasts expressing NHE1 alanine mutants for either Rock (T653A) or ribosomal S6 kinase (Rsk; S703A) we show that each site is partially responsible for the LPA-induced increase in transport activity while NHE1 phosphorylation by either Rock or Rsk at their respective site is sufficient for LPA stimulated stress fiber formation and migration. Furthermore, mutation of either T653 or S703 leads to a higher basal pH level and a significantly higher proliferation rate. Our results identify the direct phosphorylation of NHE1 by Rock and suggest that both RhoA and Ras pathways mediate NHE1-dependent ion transport and migration in fibroblasts.

Original languageEnglish (US)
Pages (from-to)498-509
Number of pages12
JournalCellular Signalling
Volume27
Issue number3
DOIs
StatePublished - Mar 1 2015

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90-kDa Ribosomal Protein S6 Kinases
Sodium-Hydrogen Antiporter
Phosphotransferases
Ion Transport
Phosphorylation
Fibroblasts
Ribosomal Protein S6 Kinases
Stress Fibers
Threonine
Alanine
Cell Movement
Mass Spectrometry
Intercellular Signaling Peptides and Proteins
Protein Isoforms
Mutation
lysophosphatidic acid

Keywords

  • Cytoskeleton
  • Lysophosphatidic acid
  • NHE1
  • Rock kinase
  • Rsk
  • Sodium hydrogen exchanger

ASJC Scopus subject areas

  • Cell Biology

Cite this

RhoA Kinase (Rock) and p90 Ribosomal S6 Kinase (p90Rsk) phosphorylation of the sodium hydrogen exchanger (NHE1) is required for lysophosphatidic acid-induced transport, cytoskeletal organization and migration. / Wallert, Mark A.; Hammes, Daniel; Nguyen, Tony; Kiefer, Lea; Berthelsen, Nick; Kern, Andrew; Anderson-Tiege, Kristina; Shabb, John B.; Muhonen, Wallace W.; Grove, Bryon D.; Provost, Joseph J.

In: Cellular Signalling, Vol. 27, No. 3, 01.03.2015, p. 498-509.

Research output: Contribution to journalArticle

Wallert, Mark A. ; Hammes, Daniel ; Nguyen, Tony ; Kiefer, Lea ; Berthelsen, Nick ; Kern, Andrew ; Anderson-Tiege, Kristina ; Shabb, John B. ; Muhonen, Wallace W. ; Grove, Bryon D. ; Provost, Joseph J. / RhoA Kinase (Rock) and p90 Ribosomal S6 Kinase (p90Rsk) phosphorylation of the sodium hydrogen exchanger (NHE1) is required for lysophosphatidic acid-induced transport, cytoskeletal organization and migration. In: Cellular Signalling. 2015 ; Vol. 27, No. 3. pp. 498-509.
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