Rhabdomyosarcoma-derived cell lines exhibit aberrant expression of the cell-cell adhesion molecules N-CAM, N-cadherin, and cadherin-associated proteins

Alejandro Peralta Soler, Keith R. Johnson, Margaret J. Wheelock, Karen A. Knudsen

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Many cancer cells show aberrant adhesion properties that likely contribute to tumorigenesis, invasion, and metastasis. Here, we examine three MyoD-expressing, rhabdomyosarcoma-derived human cell lines (RD, A-204, and HS 729) for their expression of the neural cell adhesion molecule (N-CAM), N-cadherin, and the cadherin-associated proteins, α-catenin, β-catenin, and plakoglobin, using specific antibodies and immunoblotting and immunocytochemical methods. Normally, during the formation of skeletal muscle, both N-CAM and N-cadherin are expressed and participate in mediating myoblast adhesion accompanying cell fusion. RD cells express N-CAM, N-cadherin, and the cadherin-associated proteins; however, N-CAM is expressed as a highly sialylated isoform that functions poorly in promoting Ca2+-independent cell aggregation. HS 729 cells express N-cadherin and its associated intracellular proteins but have no detectable N-CAM. A-204 cells express no detectable N-CAM or N-cadherin but do express cadherin-associated proteins. Thus, all three rhabdomyosarcoma cell lines exhibit some abnormality in the expression of adhesion molecules known to participate in skeletal myogenosis; however, no common defect was observed that might be considered as a characteristic marker for rhabdomyosarcomas.

Original languageEnglish (US)
Pages (from-to)84-93
Number of pages10
JournalExperimental Cell Research
Volume208
Issue number1
DOIs
StatePublished - Sep 1993

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Neural Cell Adhesion Molecules
Rhabdomyosarcoma
Cell Adhesion Molecules
Cadherins
Cell Line
A 204
Proteins
Catenins
gamma Catenin
Cell Aggregation
Cell Fusion
Myoblasts
Immunoblotting
Protein Isoforms
Carcinogenesis
Skeletal Muscle
Neoplasm Metastasis
Antibodies

ASJC Scopus subject areas

  • Cell Biology

Cite this

Rhabdomyosarcoma-derived cell lines exhibit aberrant expression of the cell-cell adhesion molecules N-CAM, N-cadherin, and cadherin-associated proteins. / Soler, Alejandro Peralta; Johnson, Keith R.; Wheelock, Margaret J.; Knudsen, Karen A.

In: Experimental Cell Research, Vol. 208, No. 1, 09.1993, p. 84-93.

Research output: Contribution to journalArticle

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