Reversible papillary hyperplasia of the rat urinary bladder

T. Shirai, Samuel Monroe Cohen, S. Fukushima, M. Hananouchi, N. Ito

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

A rapid, reproducible method for the production of ulcers and reversible regenerative hyperplasia of the urinary bladder of rats is described. This method does not involve administration of a toxic chemical or retention of a foreign body. Ulceration was produced by means of applying a steel rod, 5 mm in diameter, frozen at -78C, to the serosal surface of the bladder for 2 seconds, twice, with a 5-second interval between each application. Sequential histologic observations showed that ulcers were accompanied by necrosis of the entire thickness of the bladder at the site of freezing with extensive acute inflammation. Regenerative hyperplasia was evident at the edge of the ulcer by the second day, with greatest severity at 5 days when papillary and nodular hyperplasia were present. At 15 days the bladders were normal or had only minimal hyperplasia. The labeling index by autoradiography was highest in the mildly hyperplastic area near the ulcer at 2 days and remained relatively high through 5 days. The hyperplastic epithelium surrounding the ulcer observed by scanning electron microscopy had numerous degenerative cells on the surface, and small epithelial cells with numerous short, uniform microvilli on their luminal surface were also observed. The microvilli had a symmetric luminal membrane, as observed on transmission electron microscopy. More peripherally the cells had microridges only or microvilli and microridges on their luminal surface. Neither pleomorphic microvilli nor a structured glycocalyx fuzz on microvilli was observed during the process of regenerative hyperplasia, distinguishing it from neoplastic bladder proliferations.

Original languageEnglish (US)
Pages (from-to)33-44
Number of pages12
JournalAmerican Journal of Pathology
Volume91
Issue number1
StatePublished - Nov 13 1978

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Microvilli
Ulcer
Hyperplasia
Urinary Bladder
Glycocalyx
Poisons
Steel
Foreign Bodies
Autoradiography
Transmission Electron Microscopy
Electron Scanning Microscopy
Freezing
Necrosis
Epithelium
Epithelial Cells
Inflammation
Membranes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Shirai, T., Cohen, S. M., Fukushima, S., Hananouchi, M., & Ito, N. (1978). Reversible papillary hyperplasia of the rat urinary bladder. American Journal of Pathology, 91(1), 33-44.

Reversible papillary hyperplasia of the rat urinary bladder. / Shirai, T.; Cohen, Samuel Monroe; Fukushima, S.; Hananouchi, M.; Ito, N.

In: American Journal of Pathology, Vol. 91, No. 1, 13.11.1978, p. 33-44.

Research output: Contribution to journalArticle

Shirai, T, Cohen, SM, Fukushima, S, Hananouchi, M & Ito, N 1978, 'Reversible papillary hyperplasia of the rat urinary bladder', American Journal of Pathology, vol. 91, no. 1, pp. 33-44.
Shirai T, Cohen SM, Fukushima S, Hananouchi M, Ito N. Reversible papillary hyperplasia of the rat urinary bladder. American Journal of Pathology. 1978 Nov 13;91(1):33-44.
Shirai, T. ; Cohen, Samuel Monroe ; Fukushima, S. ; Hananouchi, M. ; Ito, N. / Reversible papillary hyperplasia of the rat urinary bladder. In: American Journal of Pathology. 1978 ; Vol. 91, No. 1. pp. 33-44.
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