Reversed-phase ion-pair high-performance liquid chromatographic procedure for determination of histamine and its metabolites in rat urine

Julia Y. Hui, Steve L. Taylor

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A reversed-phase ion-pair high-performance liquid chromatographic procedure for quantitative determination of histamine and its metabolites in rat urine is described. This method allows simultaneous analysis of five major histamine metabolites. Good separations were obtained by using 1-epntanesulfonic acid as the ion-pair reagent. A gradient elution program was used; the total elution time was less than 22 min. Linear standard curves with high correlation coefficients were obtained. This procedure has the advantage of requiring little sample preparation time and handling, and therefore maximizes the recovery of metabolites.

Original languageEnglish (US)
Pages (from-to)443-449
Number of pages7
JournalJournal of Chromatography A
Volume312
Issue numberC
DOIs
StatePublished - 1984

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Metabolites
Histamine
Rats
Urine
Ions
Liquids
Recovery
Acids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

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AB - A reversed-phase ion-pair high-performance liquid chromatographic procedure for quantitative determination of histamine and its metabolites in rat urine is described. This method allows simultaneous analysis of five major histamine metabolites. Good separations were obtained by using 1-epntanesulfonic acid as the ion-pair reagent. A gradient elution program was used; the total elution time was less than 22 min. Linear standard curves with high correlation coefficients were obtained. This procedure has the advantage of requiring little sample preparation time and handling, and therefore maximizes the recovery of metabolites.

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