Retroviral-infection increases tumorigenic potential of MDA-MB-231 breast carcinoma cells by expanding an aldehyde dehydrogenase (ALDH1) positive stem-cell like population

Lauren J. Wegman-Points, Melissa LT Teoh-Fitzgerald, Gaowei Mao, Yueming Zhu, Melissa A. Fath, Douglas R. Spitz, Frederick E. Domann

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Retroviral transformation has been associated with pro-proliferative oncogenic signaling in human cells. The current study demonstrates that transduction of human breast carcinoma cells (MDA-MB231) with LXSN and QCXIP retroviral vectors causes significant increases in growth rate, clonogenic fraction, and aldehyde dehydrogenase-1 positive cells (ALDH1+), which is associated with increased steady-state levels of cancer stem cell populations. Furthermore, this retroviral-induced enhancement of cancer cell growth in vitro was also accompanied by a significant increase in xenograft tumor growth rate in vivo. The retroviral induced increases in cancer cell growth rate were partially inhibited by treatment with 100 U/ml polyethylene glycol-conjugated-(PEG)-superoxide dismutase and/or PEG-catalase. These results show that retroviral infection of MDA-MB231 human breast cancer cells is capable of enhancing cell proliferation and cancer stem cell populations as well as suggesting that modulation of reactive oxygen species-induced pro-survival signaling pathways may be involved inthese effects.

Original languageEnglish (US)
Pages (from-to)847-854
Number of pages8
JournalRedox Biology
Volume2
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Aldehyde Dehydrogenase
Stem cells
Stem Cells
Cells
Breast Neoplasms
Cell growth
Infection
Population
Neoplastic Stem Cells
Growth
Cell proliferation
Heterografts
Polyethylene glycols
Superoxide Dismutase
Tumors
Reactive Oxygen Species
Neoplasms
Modulation
aldehyde dehydrogenase 1
Cell Proliferation

Keywords

  • Aldehyde dehydrogenase positive
  • Antioxidant enzymes
  • Mammary cancer
  • Oxidative stress
  • Stem cells
  • Viral carcinogenesis

ASJC Scopus subject areas

  • Organic Chemistry
  • Clinical Biochemistry

Cite this

Retroviral-infection increases tumorigenic potential of MDA-MB-231 breast carcinoma cells by expanding an aldehyde dehydrogenase (ALDH1) positive stem-cell like population. / Wegman-Points, Lauren J.; Teoh-Fitzgerald, Melissa LT; Mao, Gaowei; Zhu, Yueming; Fath, Melissa A.; Spitz, Douglas R.; Domann, Frederick E.

In: Redox Biology, Vol. 2, No. 1, 2014, p. 847-854.

Research output: Contribution to journalArticle

Wegman-Points, Lauren J. ; Teoh-Fitzgerald, Melissa LT ; Mao, Gaowei ; Zhu, Yueming ; Fath, Melissa A. ; Spitz, Douglas R. ; Domann, Frederick E. / Retroviral-infection increases tumorigenic potential of MDA-MB-231 breast carcinoma cells by expanding an aldehyde dehydrogenase (ALDH1) positive stem-cell like population. In: Redox Biology. 2014 ; Vol. 2, No. 1. pp. 847-854.
@article{43d81cd47f85494c9a2871fa5e103597,
title = "Retroviral-infection increases tumorigenic potential of MDA-MB-231 breast carcinoma cells by expanding an aldehyde dehydrogenase (ALDH1) positive stem-cell like population",
abstract = "Retroviral transformation has been associated with pro-proliferative oncogenic signaling in human cells. The current study demonstrates that transduction of human breast carcinoma cells (MDA-MB231) with LXSN and QCXIP retroviral vectors causes significant increases in growth rate, clonogenic fraction, and aldehyde dehydrogenase-1 positive cells (ALDH1+), which is associated with increased steady-state levels of cancer stem cell populations. Furthermore, this retroviral-induced enhancement of cancer cell growth in vitro was also accompanied by a significant increase in xenograft tumor growth rate in vivo. The retroviral induced increases in cancer cell growth rate were partially inhibited by treatment with 100 U/ml polyethylene glycol-conjugated-(PEG)-superoxide dismutase and/or PEG-catalase. These results show that retroviral infection of MDA-MB231 human breast cancer cells is capable of enhancing cell proliferation and cancer stem cell populations as well as suggesting that modulation of reactive oxygen species-induced pro-survival signaling pathways may be involved inthese effects.",
keywords = "Aldehyde dehydrogenase positive, Antioxidant enzymes, Mammary cancer, Oxidative stress, Stem cells, Viral carcinogenesis",
author = "Wegman-Points, {Lauren J.} and Teoh-Fitzgerald, {Melissa LT} and Gaowei Mao and Yueming Zhu and Fath, {Melissa A.} and Spitz, {Douglas R.} and Domann, {Frederick E.}",
year = "2014",
doi = "10.1016/j.redox.2014.06.006",
language = "English (US)",
volume = "2",
pages = "847--854",
journal = "Redox Biology",
issn = "2213-2317",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Retroviral-infection increases tumorigenic potential of MDA-MB-231 breast carcinoma cells by expanding an aldehyde dehydrogenase (ALDH1) positive stem-cell like population

AU - Wegman-Points, Lauren J.

AU - Teoh-Fitzgerald, Melissa LT

AU - Mao, Gaowei

AU - Zhu, Yueming

AU - Fath, Melissa A.

AU - Spitz, Douglas R.

AU - Domann, Frederick E.

PY - 2014

Y1 - 2014

N2 - Retroviral transformation has been associated with pro-proliferative oncogenic signaling in human cells. The current study demonstrates that transduction of human breast carcinoma cells (MDA-MB231) with LXSN and QCXIP retroviral vectors causes significant increases in growth rate, clonogenic fraction, and aldehyde dehydrogenase-1 positive cells (ALDH1+), which is associated with increased steady-state levels of cancer stem cell populations. Furthermore, this retroviral-induced enhancement of cancer cell growth in vitro was also accompanied by a significant increase in xenograft tumor growth rate in vivo. The retroviral induced increases in cancer cell growth rate were partially inhibited by treatment with 100 U/ml polyethylene glycol-conjugated-(PEG)-superoxide dismutase and/or PEG-catalase. These results show that retroviral infection of MDA-MB231 human breast cancer cells is capable of enhancing cell proliferation and cancer stem cell populations as well as suggesting that modulation of reactive oxygen species-induced pro-survival signaling pathways may be involved inthese effects.

AB - Retroviral transformation has been associated with pro-proliferative oncogenic signaling in human cells. The current study demonstrates that transduction of human breast carcinoma cells (MDA-MB231) with LXSN and QCXIP retroviral vectors causes significant increases in growth rate, clonogenic fraction, and aldehyde dehydrogenase-1 positive cells (ALDH1+), which is associated with increased steady-state levels of cancer stem cell populations. Furthermore, this retroviral-induced enhancement of cancer cell growth in vitro was also accompanied by a significant increase in xenograft tumor growth rate in vivo. The retroviral induced increases in cancer cell growth rate were partially inhibited by treatment with 100 U/ml polyethylene glycol-conjugated-(PEG)-superoxide dismutase and/or PEG-catalase. These results show that retroviral infection of MDA-MB231 human breast cancer cells is capable of enhancing cell proliferation and cancer stem cell populations as well as suggesting that modulation of reactive oxygen species-induced pro-survival signaling pathways may be involved inthese effects.

KW - Aldehyde dehydrogenase positive

KW - Antioxidant enzymes

KW - Mammary cancer

KW - Oxidative stress

KW - Stem cells

KW - Viral carcinogenesis

UR - http://www.scopus.com/inward/record.url?scp=84903743355&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903743355&partnerID=8YFLogxK

U2 - 10.1016/j.redox.2014.06.006

DO - 10.1016/j.redox.2014.06.006

M3 - Article

C2 - 25009786

AN - SCOPUS:84903743355

VL - 2

SP - 847

EP - 854

JO - Redox Biology

JF - Redox Biology

SN - 2213-2317

IS - 1

ER -