Retinal properties and potential of the adult mammalian ciliary epithelium stem cells

Ani V. Das, Jackson James, Jörg Rahnenführer, Wallace B Thoreson, Sumitra Bhattacharya, Xing Zhao, Iqbal Ahmad

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The ciliary epithelium (CE) in the adult mammalian eye harbors a mitotic quiescent population of neural stem cells. Here we have compared the cellular and molecular properties of CE stem cells and populations of retinal progenitors that define the early and late stages of histogenesis. The CE stem cells and retinal progenitors proliferate in the presence of mitogens and share the expression of universal neural and retinal progenitor markers. However, the expression of the majority of retinal progenitor markers (e.g., Chx10) is transient in the former when compared to the latter, in vitro. They are similar to early than late retinal progenitors in their proliferative response to FGF2 and/or EGF. Analysis of the differentiation potential of CE stem cells shows that they are capable of generating both early (e.g., retinal ganglion cells) and late (e.g., rod photoreceptors) born retinal neurons. However, under identical differentiation conditions, i.e., in the presence of 1% FBS, they generate more early-born retinal neurons than late-born retinal neurons showing a preference for generating early retinal neurons. Transcription profiling of these cells and retinal progenitors demonstrate that they share ∼80% of the expressed genes. The CE stem cells have more unique genes in common with early retinal progenitors than late retinal progenitors. Both proliferative/ differential potential and transcription profiles suggest that CE stem cells may be a residual population of stem cells of optic neuroepithelium, representing a stage antecedent to retinal progenitors.

Original languageEnglish (US)
Pages (from-to)1653-1666
Number of pages14
JournalVision Research
Volume45
Issue number13
DOIs
StatePublished - Jan 1 2005

Fingerprint

Stem Cells
Epithelium
Retinal Neurons
Population
Retinal Rod Photoreceptor Cells
Neural Stem Cells
Retinal Ganglion Cells
Fibroblast Growth Factor 2
Mitogens
Epidermal Growth Factor
Genes

Keywords

  • Ciliary epithelium
  • Progenitors
  • Retina
  • Retinal development
  • Stem cells
  • Transcriptional profiling

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Retinal properties and potential of the adult mammalian ciliary epithelium stem cells. / Das, Ani V.; James, Jackson; Rahnenführer, Jörg; Thoreson, Wallace B; Bhattacharya, Sumitra; Zhao, Xing; Ahmad, Iqbal.

In: Vision Research, Vol. 45, No. 13, 01.01.2005, p. 1653-1666.

Research output: Contribution to journalArticle

Das, Ani V. ; James, Jackson ; Rahnenführer, Jörg ; Thoreson, Wallace B ; Bhattacharya, Sumitra ; Zhao, Xing ; Ahmad, Iqbal. / Retinal properties and potential of the adult mammalian ciliary epithelium stem cells. In: Vision Research. 2005 ; Vol. 45, No. 13. pp. 1653-1666.
@article{17fade213d834077bd36bb8c147ea64a,
title = "Retinal properties and potential of the adult mammalian ciliary epithelium stem cells",
abstract = "The ciliary epithelium (CE) in the adult mammalian eye harbors a mitotic quiescent population of neural stem cells. Here we have compared the cellular and molecular properties of CE stem cells and populations of retinal progenitors that define the early and late stages of histogenesis. The CE stem cells and retinal progenitors proliferate in the presence of mitogens and share the expression of universal neural and retinal progenitor markers. However, the expression of the majority of retinal progenitor markers (e.g., Chx10) is transient in the former when compared to the latter, in vitro. They are similar to early than late retinal progenitors in their proliferative response to FGF2 and/or EGF. Analysis of the differentiation potential of CE stem cells shows that they are capable of generating both early (e.g., retinal ganglion cells) and late (e.g., rod photoreceptors) born retinal neurons. However, under identical differentiation conditions, i.e., in the presence of 1{\%} FBS, they generate more early-born retinal neurons than late-born retinal neurons showing a preference for generating early retinal neurons. Transcription profiling of these cells and retinal progenitors demonstrate that they share ∼80{\%} of the expressed genes. The CE stem cells have more unique genes in common with early retinal progenitors than late retinal progenitors. Both proliferative/ differential potential and transcription profiles suggest that CE stem cells may be a residual population of stem cells of optic neuroepithelium, representing a stage antecedent to retinal progenitors.",
keywords = "Ciliary epithelium, Progenitors, Retina, Retinal development, Stem cells, Transcriptional profiling",
author = "Das, {Ani V.} and Jackson James and J{\"o}rg Rahnenf{\"u}hrer and Thoreson, {Wallace B} and Sumitra Bhattacharya and Xing Zhao and Iqbal Ahmad",
year = "2005",
month = "1",
day = "1",
doi = "10.1016/j.visres.2004.12.017",
language = "English (US)",
volume = "45",
pages = "1653--1666",
journal = "Vision Research",
issn = "0042-6989",
publisher = "Elsevier Limited",
number = "13",

}

TY - JOUR

T1 - Retinal properties and potential of the adult mammalian ciliary epithelium stem cells

AU - Das, Ani V.

AU - James, Jackson

AU - Rahnenführer, Jörg

AU - Thoreson, Wallace B

AU - Bhattacharya, Sumitra

AU - Zhao, Xing

AU - Ahmad, Iqbal

PY - 2005/1/1

Y1 - 2005/1/1

N2 - The ciliary epithelium (CE) in the adult mammalian eye harbors a mitotic quiescent population of neural stem cells. Here we have compared the cellular and molecular properties of CE stem cells and populations of retinal progenitors that define the early and late stages of histogenesis. The CE stem cells and retinal progenitors proliferate in the presence of mitogens and share the expression of universal neural and retinal progenitor markers. However, the expression of the majority of retinal progenitor markers (e.g., Chx10) is transient in the former when compared to the latter, in vitro. They are similar to early than late retinal progenitors in their proliferative response to FGF2 and/or EGF. Analysis of the differentiation potential of CE stem cells shows that they are capable of generating both early (e.g., retinal ganglion cells) and late (e.g., rod photoreceptors) born retinal neurons. However, under identical differentiation conditions, i.e., in the presence of 1% FBS, they generate more early-born retinal neurons than late-born retinal neurons showing a preference for generating early retinal neurons. Transcription profiling of these cells and retinal progenitors demonstrate that they share ∼80% of the expressed genes. The CE stem cells have more unique genes in common with early retinal progenitors than late retinal progenitors. Both proliferative/ differential potential and transcription profiles suggest that CE stem cells may be a residual population of stem cells of optic neuroepithelium, representing a stage antecedent to retinal progenitors.

AB - The ciliary epithelium (CE) in the adult mammalian eye harbors a mitotic quiescent population of neural stem cells. Here we have compared the cellular and molecular properties of CE stem cells and populations of retinal progenitors that define the early and late stages of histogenesis. The CE stem cells and retinal progenitors proliferate in the presence of mitogens and share the expression of universal neural and retinal progenitor markers. However, the expression of the majority of retinal progenitor markers (e.g., Chx10) is transient in the former when compared to the latter, in vitro. They are similar to early than late retinal progenitors in their proliferative response to FGF2 and/or EGF. Analysis of the differentiation potential of CE stem cells shows that they are capable of generating both early (e.g., retinal ganglion cells) and late (e.g., rod photoreceptors) born retinal neurons. However, under identical differentiation conditions, i.e., in the presence of 1% FBS, they generate more early-born retinal neurons than late-born retinal neurons showing a preference for generating early retinal neurons. Transcription profiling of these cells and retinal progenitors demonstrate that they share ∼80% of the expressed genes. The CE stem cells have more unique genes in common with early retinal progenitors than late retinal progenitors. Both proliferative/ differential potential and transcription profiles suggest that CE stem cells may be a residual population of stem cells of optic neuroepithelium, representing a stage antecedent to retinal progenitors.

KW - Ciliary epithelium

KW - Progenitors

KW - Retina

KW - Retinal development

KW - Stem cells

KW - Transcriptional profiling

UR - http://www.scopus.com/inward/record.url?scp=15544383505&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15544383505&partnerID=8YFLogxK

U2 - 10.1016/j.visres.2004.12.017

DO - 10.1016/j.visres.2004.12.017

M3 - Article

VL - 45

SP - 1653

EP - 1666

JO - Vision Research

JF - Vision Research

SN - 0042-6989

IS - 13

ER -