Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors

Iqbal Ahmad, Trese Leinders-Zufall, Jeffery D. Kocsis, Gordon M. Shepherd, Frank Zufall, Colin J. Barnstable

Research output: Contribution to journalArticle

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Abstract

We have identified a putative cGMP-gated cation conductance in rat retinal ganglion cells. Both in situ hybridization and polymerase chain reaction amplification detected transcripts in ganglion cells that were highly homologous to the cGMP-gated cation channel expressed in rod photoreceptors. Whole-cell patch-clamp recordings detected a current stimulated by cGMP due to activation of nonselective cation channels. This current had a reversal potential near 0 mV, showed some outward rectification, and could be blocked by Cd 2+ . The current could also be activated by a phosphodiesterase inhibitor and the nitric oxide donors sodium nitroprusside and S-nitrosocysteine. We propose that nitric oxide released from an identified subpopulation of amacrine cells may activate this channel to modulate ganglion cell activity.

Original languageEnglish (US)
Pages (from-to)155-165
Number of pages11
JournalNeuron
Volume12
Issue number1
DOIs
StatePublished - Jan 1 1994

Fingerprint

Nitric Oxide Donors
Retinal Ganglion Cells
Cations
Ganglia
Retinal Rod Photoreceptor Cells
Amacrine Cells
Phosphodiesterase Inhibitors
Nitroprusside
In Situ Hybridization
Nitric Oxide
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ahmad, I., Leinders-Zufall, T., Kocsis, J. D., Shepherd, G. M., Zufall, F., & Barnstable, C. J. (1994). Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors. Neuron, 12(1), 155-165. https://doi.org/10.1016/0896-6273(94)90160-0

Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors. / Ahmad, Iqbal; Leinders-Zufall, Trese; Kocsis, Jeffery D.; Shepherd, Gordon M.; Zufall, Frank; Barnstable, Colin J.

In: Neuron, Vol. 12, No. 1, 01.01.1994, p. 155-165.

Research output: Contribution to journalArticle

Ahmad, I, Leinders-Zufall, T, Kocsis, JD, Shepherd, GM, Zufall, F & Barnstable, CJ 1994, 'Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors', Neuron, vol. 12, no. 1, pp. 155-165. https://doi.org/10.1016/0896-6273(94)90160-0
Ahmad, Iqbal ; Leinders-Zufall, Trese ; Kocsis, Jeffery D. ; Shepherd, Gordon M. ; Zufall, Frank ; Barnstable, Colin J. / Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors. In: Neuron. 1994 ; Vol. 12, No. 1. pp. 155-165.
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