Resveratrol prevents estrogen-DNA adduct formation and neoplastic transformation in MCF-10F cells

Fang Lu, Muhammad Zahid, Cheng Wang, Muhammad Saeed, Ercole Cavalieri, Eleanor G Rogan

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Exposure to estrogens is a risk factor for breast cancer. Specific estrogen metabolites may initiate breast cancer and other cancers. Genotoxicity may be caused by cytochrome P450 (CYP)-mediated oxidation of catechol estrogens to quinones that react with DNA to form depurinating estrogen-DNA adducts. CYP1B1 favors quinone formation by catalyzing estrogen 4-hydroxylation, whereas NAD(P)H quinone oxidoreductase 1 (NQO1) catalyzes the protective reduction of quinonesto catechols. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces CYP1B1 expression through the aryl hydrocarbon receptor (AhR). Resveratrol hasanticancer effectsin diverse in vitro and in vivo systems and is an AhR antagonist that decreases CYP expression but induces NQO1 expression. The chemopreventive effect of resveratrol on breast cancer initiation was investigated in MCF-10F cells. Its effects on estrogen metabolism and formation of estrogen-DNA adducts were analyzed in culture medium by high-performance liquid chromatography, whereas its effects on CYP1B1 and NQO1 were determined by immunoblotting and immunostaining. The antitransformation effects of resveratrol were also examined. TCDD induced expression of CYP1B1 and its redistribution in the nucleusan d cytoplasm. Concomitant treatment with resveratrol dose-dependently suppressed TCDD-induced expression of CYP1B1, mainly in the cytoplasm. Resveratrol dose- and time-dependently induced expression of NQO1. NQO1 is mainly in the perinuclear membrane of control cells, but resveratrol induced NQO1 and its intracellular redistribution, which involvesnuclear translocation of nuclear factor erythroid 2-related factor 2. Resveratrol decreased estrogen metabolism and blocked formation of DNA adducts in cells treated with TCDD and/or estradiol. Resveratrol also suppressed TCDD and/or estradiol-induced cell transformation. Thus, resveratrol can prevent breast cancer initiation by blocking multiple sites in the estrogen genotoxicity pathway.

Original languageEnglish (US)
Pages (from-to)135-145
Number of pages11
JournalCancer Prevention Research
Volume1
Issue number2
DOIs
StatePublished - Jul 1 2008

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DNA Adducts
Estrogens
Breast Neoplasms
Aryl Hydrocarbon Receptors
Cytochrome P-450 Enzyme System
Estradiol
Cytoplasm
Catechol Estrogens
Catechols
resveratrol
Quinones
Hydroxylation
Immunoblotting
NAD
Culture Media
Oxidoreductases
High Pressure Liquid Chromatography
Cell Membrane
Polychlorinated Dibenzodioxins
DNA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Resveratrol prevents estrogen-DNA adduct formation and neoplastic transformation in MCF-10F cells. / Lu, Fang; Zahid, Muhammad; Wang, Cheng; Saeed, Muhammad; Cavalieri, Ercole; Rogan, Eleanor G.

In: Cancer Prevention Research, Vol. 1, No. 2, 01.07.2008, p. 135-145.

Research output: Contribution to journalArticle

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