Resveratrol, a natural product derived from grape, exhibits antiestrogenic activity and inhibits the growth of human breast cancer cells

Runqing Lu, Ginette Serrero

Research output: Contribution to journalArticle

287 Citations (Scopus)

Abstract

Resveratrol is a natural phytoalexin compound found in grapes and other food products. In this study, the effect of resveratrol on the growth of human breast cancer cells was examined. Results show that resveratrol inhibits the growth of estrogen receptor(ER)-positive MCF-7 cells in a dose- dependent fashion. Detailed studies with MCF-7 cells demonstrate that resveratrol antagonized the growth-promoting effect of 17-β-estradiol (E2) in a dose-dependent fashion at both the cellular (cell growth) and the molecular (gene activation) levels. At 5 x 10-6 M, resveratrol abolished the growth-stimulatory effect mediated by concentrations of E2 up to 10-9 M. The antiestrogenic effect of resveratrol could be observed at a concentration of 10-6 M and above. The antiestrogenic effect of resveratrol was also demonstrated at the molecular level. Resveratrol in a dose-dependent fashion antagonized the stimulation by E2 of progesterone receptor gene expression in MCF-7 cells. Moreover, expression of transforming growth factor-α and insulin-like growth factor I receptor mRNA was inhibited while the expression of transforming growth factor β2 mRNA was significantly elevated in MCF-7 cells cultivated in the presence of resveratrol (10-5 M). In summary, our results show that resveratrol, a partial ER agonist itself, acts as an ER antagonist in the presence of estrogen leading to inhibition of human breast cancer cells.

Original languageEnglish (US)
Pages (from-to)297-304
Number of pages8
JournalJournal of Cellular Physiology
Volume179
Issue number3
DOIs
StatePublished - May 10 1999

Fingerprint

Vitis
Biological Products
Cells
Breast Neoplasms
Growth
MCF-7 Cells
Transforming Growth Factors
Estrogens
resveratrol
IGF Type 1 Receptor
Messenger RNA
Cell growth
Progesterone Receptors
Gene expression
Estrogen Receptors
Transcriptional Activation
Estradiol
Genes
Chemical activation
Gene Expression

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Resveratrol, a natural product derived from grape, exhibits antiestrogenic activity and inhibits the growth of human breast cancer cells. / Lu, Runqing; Serrero, Ginette.

In: Journal of Cellular Physiology, Vol. 179, No. 3, 10.05.1999, p. 297-304.

Research output: Contribution to journalArticle

@article{33a80e8405164e238c3789b211175e3e,
title = "Resveratrol, a natural product derived from grape, exhibits antiestrogenic activity and inhibits the growth of human breast cancer cells",
abstract = "Resveratrol is a natural phytoalexin compound found in grapes and other food products. In this study, the effect of resveratrol on the growth of human breast cancer cells was examined. Results show that resveratrol inhibits the growth of estrogen receptor(ER)-positive MCF-7 cells in a dose- dependent fashion. Detailed studies with MCF-7 cells demonstrate that resveratrol antagonized the growth-promoting effect of 17-β-estradiol (E2) in a dose-dependent fashion at both the cellular (cell growth) and the molecular (gene activation) levels. At 5 x 10-6 M, resveratrol abolished the growth-stimulatory effect mediated by concentrations of E2 up to 10-9 M. The antiestrogenic effect of resveratrol could be observed at a concentration of 10-6 M and above. The antiestrogenic effect of resveratrol was also demonstrated at the molecular level. Resveratrol in a dose-dependent fashion antagonized the stimulation by E2 of progesterone receptor gene expression in MCF-7 cells. Moreover, expression of transforming growth factor-α and insulin-like growth factor I receptor mRNA was inhibited while the expression of transforming growth factor β2 mRNA was significantly elevated in MCF-7 cells cultivated in the presence of resveratrol (10-5 M). In summary, our results show that resveratrol, a partial ER agonist itself, acts as an ER antagonist in the presence of estrogen leading to inhibition of human breast cancer cells.",
author = "Runqing Lu and Ginette Serrero",
year = "1999",
month = "5",
day = "10",
doi = "10.1002/(SICI)1097-4652(199906)179:3<297::AID-JCP7>3.0.CO;2-P",
language = "English (US)",
volume = "179",
pages = "297--304",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Resveratrol, a natural product derived from grape, exhibits antiestrogenic activity and inhibits the growth of human breast cancer cells

AU - Lu, Runqing

AU - Serrero, Ginette

PY - 1999/5/10

Y1 - 1999/5/10

N2 - Resveratrol is a natural phytoalexin compound found in grapes and other food products. In this study, the effect of resveratrol on the growth of human breast cancer cells was examined. Results show that resveratrol inhibits the growth of estrogen receptor(ER)-positive MCF-7 cells in a dose- dependent fashion. Detailed studies with MCF-7 cells demonstrate that resveratrol antagonized the growth-promoting effect of 17-β-estradiol (E2) in a dose-dependent fashion at both the cellular (cell growth) and the molecular (gene activation) levels. At 5 x 10-6 M, resveratrol abolished the growth-stimulatory effect mediated by concentrations of E2 up to 10-9 M. The antiestrogenic effect of resveratrol could be observed at a concentration of 10-6 M and above. The antiestrogenic effect of resveratrol was also demonstrated at the molecular level. Resveratrol in a dose-dependent fashion antagonized the stimulation by E2 of progesterone receptor gene expression in MCF-7 cells. Moreover, expression of transforming growth factor-α and insulin-like growth factor I receptor mRNA was inhibited while the expression of transforming growth factor β2 mRNA was significantly elevated in MCF-7 cells cultivated in the presence of resveratrol (10-5 M). In summary, our results show that resveratrol, a partial ER agonist itself, acts as an ER antagonist in the presence of estrogen leading to inhibition of human breast cancer cells.

AB - Resveratrol is a natural phytoalexin compound found in grapes and other food products. In this study, the effect of resveratrol on the growth of human breast cancer cells was examined. Results show that resveratrol inhibits the growth of estrogen receptor(ER)-positive MCF-7 cells in a dose- dependent fashion. Detailed studies with MCF-7 cells demonstrate that resveratrol antagonized the growth-promoting effect of 17-β-estradiol (E2) in a dose-dependent fashion at both the cellular (cell growth) and the molecular (gene activation) levels. At 5 x 10-6 M, resveratrol abolished the growth-stimulatory effect mediated by concentrations of E2 up to 10-9 M. The antiestrogenic effect of resveratrol could be observed at a concentration of 10-6 M and above. The antiestrogenic effect of resveratrol was also demonstrated at the molecular level. Resveratrol in a dose-dependent fashion antagonized the stimulation by E2 of progesterone receptor gene expression in MCF-7 cells. Moreover, expression of transforming growth factor-α and insulin-like growth factor I receptor mRNA was inhibited while the expression of transforming growth factor β2 mRNA was significantly elevated in MCF-7 cells cultivated in the presence of resveratrol (10-5 M). In summary, our results show that resveratrol, a partial ER agonist itself, acts as an ER antagonist in the presence of estrogen leading to inhibition of human breast cancer cells.

UR - http://www.scopus.com/inward/record.url?scp=0032913414&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032913414&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-4652(199906)179:3<297::AID-JCP7>3.0.CO;2-P

DO - 10.1002/(SICI)1097-4652(199906)179:3<297::AID-JCP7>3.0.CO;2-P

M3 - Article

C2 - 10228948

AN - SCOPUS:0032913414

VL - 179

SP - 297

EP - 304

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 3

ER -