Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga

Yunnan Xia, Dwight E. Burbank, James L. van Etten

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A type II restriction endonuclease, CviBI, was isolated from a eukaryotic, Chlorella-like green alga infected with the dsDNA containing virus NC-1A. The enzyme recognizes the sequence GANTC and cleaves DNA between the G and A. Methylation of deoxyadenosine in the GANTC sequence probably inhibits enzyme activity. In vitro CviBI cleaves host nuclear DNA but not viral DNA. A survey of 18 other viruses which infect the same Chlorella sp. revealed that infection with 5 of these viruses also induced a restriction endonuclease which cleaves DNA into the same size fragments as CviBI.

Original languageEnglish (US)
Pages (from-to)6017-6030
Number of pages14
JournalNucleic acids research
Volume14
Issue number15
DOIs
StatePublished - Aug 11 1986

Fingerprint

Green Algae
Chlorella
Chlorophyta
DNA Restriction Enzymes
Virus Diseases
Algae
Viruses
Virus
Infection
DNA
Restriction
Enzymes
Type II Site Specific Deoxyribonucleases
Methylation
Viral DNA
Enzyme activity
Fragment
Endonucleases

ASJC Scopus subject areas

  • Genetics

Cite this

Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga. / Xia, Yunnan; Burbank, Dwight E.; van Etten, James L.

In: Nucleic acids research, Vol. 14, No. 15, 11.08.1986, p. 6017-6030.

Research output: Contribution to journalArticle

@article{2c524bf4fb94496a8598dd3eb9675a69,
title = "Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga",
abstract = "A type II restriction endonuclease, CviBI, was isolated from a eukaryotic, Chlorella-like green alga infected with the dsDNA containing virus NC-1A. The enzyme recognizes the sequence GANTC and cleaves DNA between the G and A. Methylation of deoxyadenosine in the GANTC sequence probably inhibits enzyme activity. In vitro CviBI cleaves host nuclear DNA but not viral DNA. A survey of 18 other viruses which infect the same Chlorella sp. revealed that infection with 5 of these viruses also induced a restriction endonuclease which cleaves DNA into the same size fragments as CviBI.",
author = "Yunnan Xia and Burbank, {Dwight E.} and {van Etten}, {James L.}",
year = "1986",
month = "8",
day = "11",
doi = "10.1093/nar/14.15.6017",
language = "English (US)",
volume = "14",
pages = "6017--6030",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "15",

}

TY - JOUR

T1 - Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga

AU - Xia, Yunnan

AU - Burbank, Dwight E.

AU - van Etten, James L.

PY - 1986/8/11

Y1 - 1986/8/11

N2 - A type II restriction endonuclease, CviBI, was isolated from a eukaryotic, Chlorella-like green alga infected with the dsDNA containing virus NC-1A. The enzyme recognizes the sequence GANTC and cleaves DNA between the G and A. Methylation of deoxyadenosine in the GANTC sequence probably inhibits enzyme activity. In vitro CviBI cleaves host nuclear DNA but not viral DNA. A survey of 18 other viruses which infect the same Chlorella sp. revealed that infection with 5 of these viruses also induced a restriction endonuclease which cleaves DNA into the same size fragments as CviBI.

AB - A type II restriction endonuclease, CviBI, was isolated from a eukaryotic, Chlorella-like green alga infected with the dsDNA containing virus NC-1A. The enzyme recognizes the sequence GANTC and cleaves DNA between the G and A. Methylation of deoxyadenosine in the GANTC sequence probably inhibits enzyme activity. In vitro CviBI cleaves host nuclear DNA but not viral DNA. A survey of 18 other viruses which infect the same Chlorella sp. revealed that infection with 5 of these viruses also induced a restriction endonuclease which cleaves DNA into the same size fragments as CviBI.

UR - http://www.scopus.com/inward/record.url?scp=0023046936&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023046936&partnerID=8YFLogxK

U2 - 10.1093/nar/14.15.6017

DO - 10.1093/nar/14.15.6017

M3 - Article

C2 - 3018667

AN - SCOPUS:0023046936

VL - 14

SP - 6017

EP - 6030

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 15

ER -