Restoration of T and NK cell function in GM-CSF mobilized stem cell products from breast cancer patients by monocyte depletion

A. G. Ageitos, K. Ino, I. Ozerol, S. Tarantolo, D. G. Heimann, James E Talmadge

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Rapid immune reconstitution is observed following autologous peripheral blood stem cell transplantation (PSCT) as compared to autologous bone marrow transplantation (ABMT), although it is depressed compared to that observed in normal individuals. The immune dysfunction occurs despite the restoration of normal lymphoid cell numbers and may be associated with the immunologic characteristics of the infused peripheral blood stem cell (PSC) product. We report herein that the in vitro T cell proliferation and NK activity in PSC products of breast cancer patients are significantly increased following the removal of CD14+ monocytes (33 ± 2% of the PSC product) by carbonyl iron magnetic cell isolation (CI). In vitro expansion of PSC cells cultured for 7-21 days in the presence of interleukin-2 (IL-2) is also significantly increased by depletion of the phagocytic cells. The PHA and IL-2 mitogenic responses, as well as NK activity of the expanded cells, was also significantly increased by the depletion of the phagocytes. In summary, the depletion of phagocytic monocytes from PSC products restores the proliferative and functional properties of T and NK lymphocytes and may facilitate adoptive cellular therapy, as well as rapid immunologic reconstitution post-PSCT.

Original languageEnglish (US)
Pages (from-to)117-123
Number of pages7
JournalBone marrow transplantation
Volume20
Issue number2
DOIs
StatePublished - Jul 2 1997

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Granulocyte-Macrophage Colony-Stimulating Factor
Natural Killer Cells
Monocytes
Stem Cells
Breast Neoplasms
T-Lymphocytes
Peripheral Blood Stem Cell Transplantation
Phagocytes
Interleukin-2
Lymphocytes
Cell Separation
Autologous Transplantation
Bone Marrow Transplantation
Cultured Cells
Iron
Cell Count
Cell Proliferation
Peripheral Blood Stem Cells
In Vitro Techniques
Therapeutics

Keywords

  • Monocytes
  • NK
  • Suppression
  • T cell
  • Tolerance

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Restoration of T and NK cell function in GM-CSF mobilized stem cell products from breast cancer patients by monocyte depletion. / Ageitos, A. G.; Ino, K.; Ozerol, I.; Tarantolo, S.; Heimann, D. G.; Talmadge, James E.

In: Bone marrow transplantation, Vol. 20, No. 2, 02.07.1997, p. 117-123.

Research output: Contribution to journalArticle

Ageitos, A. G. ; Ino, K. ; Ozerol, I. ; Tarantolo, S. ; Heimann, D. G. ; Talmadge, James E. / Restoration of T and NK cell function in GM-CSF mobilized stem cell products from breast cancer patients by monocyte depletion. In: Bone marrow transplantation. 1997 ; Vol. 20, No. 2. pp. 117-123.
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