Resistance of soil-bound prions to rumen digestion

Samuel E. Saunders, Shannon L Bartelt-Hunt, Jason C. Bartz

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Before prion uptake and infection can occur in the lower gastrointestinal system, ingested prions are subjected to anaerobic digestion in the rumen of cervids and bovids. The susceptibility of soil-bound prions to rumen digestion has not been evaluated previously. In this study, prions from infectious brain homogenates as well as prions bound to a range of soils and soil minerals were subjected to in vitro rumen digestion, and changes in PrP levels were measured via western blot. Binding to clay appeared to protect noninfectious hamster PrPc from complete digestion, while both unbound and soil-bound infectious PrPSc proved highly resistant to rumen digestion. In addition, no change in intracerebral incubation period was observed following active rumen digestion of unbound hamster HY TME prions and HY TME prions bound to a silty clay loam soil. These results demonstrate that both unbound and soil-bound prions readily survive rumen digestion without a reduction in infectivity, further supporting the potential for soil-mediated transmission of chronic wasting disease (CWD) and scrapie in the environment.

Original languageEnglish (US)
Article numbere44051
JournalPloS one
Volume7
Issue number8
DOIs
StatePublished - Aug 24 2012

Fingerprint

Prions
prions
Rumen
rumen fermentation
Digestion
Soil
Soils
soil
hamsters
Cricetinae
Chronic Wasting Disease
chronic wasting disease
PrPSc proteins
Scrapie
Anaerobic digestion
anaerobic digestion
scrapie
gastrointestinal system
Minerals
rumen

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Resistance of soil-bound prions to rumen digestion. / Saunders, Samuel E.; Bartelt-Hunt, Shannon L; Bartz, Jason C.

In: PloS one, Vol. 7, No. 8, e44051, 24.08.2012.

Research output: Contribution to journalArticle

Saunders, Samuel E. ; Bartelt-Hunt, Shannon L ; Bartz, Jason C. / Resistance of soil-bound prions to rumen digestion. In: PloS one. 2012 ; Vol. 7, No. 8.
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