Requirements for T lymphocyte migration in explanted lymph nodes

Julie H. Huang, L. Isabel Cárdenas-Navia, Charles C. Caldwell, Troy J Plumb, Caius G. Radu, Paulo N. Rocha, Tuere Wilder, Jonathan S. Bromberg, Bruce N. Cronstein, Michail Sitkovsky, Mark W. Dewhirst, Michael L. Dustin

Research output: Contribution to journalArticle

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Abstract

Although the requirements for T lymphocyte homing to lymph nodes (LNs) are well studied, much less is known about the requirements for T lymphocyte locomotion within LNs. Imaging of murine T lymphocyte migration in explanted LNs using two-photon laser-scanning fluorescence microscopy provides an opportunity to systematically study these requirements. We have developed a closed system for imaging an intact LN with controlled temperature, oxygenation, and perfusion rate. Naive T lymphocyte locomotion in the deep paracortex of the LN required a perfusion rate of > 13 μm/s and a partial pressure of O2 (pO2) of > 7.4%. Naive T lymphocyte locomotion in the subcapsular region was 38% slower and had higher turning angles and arrest coefficients than naive T lymphocytes in the deep paracortex. T lymphocyte activation decreased the requirement for pO2, but also decreased the speed of locomotion in the deep paracortex. Although CCR7-/- naive T cells displayed a small reduction in locomotion, systemic treatment with pertussis toxin reduced naive T lymphocyte speed by 59%, indicating a contribution of Gαi-mediated signaling, but involvement of other G protein-coupled receptors besides CCR7. Receptor knockouts or pharmacological inhibition in the adenosine, PG/lipoxygenase, lysophosphatidylcholine, and sphingosine-1-phosphate pathways did not individually alter naive T cell migration. These data implicate pO2, tissue architecture, and G-protein coupled receptor signaling in regulation of naive T lymphocyte migration in explanted LNs.

Original languageEnglish (US)
Pages (from-to)7747-7755
Number of pages9
JournalJournal of Immunology
Volume178
Issue number12
DOIs
StatePublished - Jun 15 2007

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Lymph Nodes
T-Lymphocytes
Locomotion
G-Protein-Coupled Receptors
Perfusion
Pressure
Lysophosphatidylcholines
Lipoxygenase
Partial Pressure
Pertussis Toxin
Lymphocyte Activation
Photons
Fluorescence Microscopy
Confocal Microscopy
Adenosine
Cell Movement
Pharmacology
Temperature

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Huang, J. H., Cárdenas-Navia, L. I., Caldwell, C. C., Plumb, T. J., Radu, C. G., Rocha, P. N., ... Dustin, M. L. (2007). Requirements for T lymphocyte migration in explanted lymph nodes. Journal of Immunology, 178(12), 7747-7755. https://doi.org/10.4049/jimmunol.178.12.7747

Requirements for T lymphocyte migration in explanted lymph nodes. / Huang, Julie H.; Cárdenas-Navia, L. Isabel; Caldwell, Charles C.; Plumb, Troy J; Radu, Caius G.; Rocha, Paulo N.; Wilder, Tuere; Bromberg, Jonathan S.; Cronstein, Bruce N.; Sitkovsky, Michail; Dewhirst, Mark W.; Dustin, Michael L.

In: Journal of Immunology, Vol. 178, No. 12, 15.06.2007, p. 7747-7755.

Research output: Contribution to journalArticle

Huang, JH, Cárdenas-Navia, LI, Caldwell, CC, Plumb, TJ, Radu, CG, Rocha, PN, Wilder, T, Bromberg, JS, Cronstein, BN, Sitkovsky, M, Dewhirst, MW & Dustin, ML 2007, 'Requirements for T lymphocyte migration in explanted lymph nodes', Journal of Immunology, vol. 178, no. 12, pp. 7747-7755. https://doi.org/10.4049/jimmunol.178.12.7747
Huang JH, Cárdenas-Navia LI, Caldwell CC, Plumb TJ, Radu CG, Rocha PN et al. Requirements for T lymphocyte migration in explanted lymph nodes. Journal of Immunology. 2007 Jun 15;178(12):7747-7755. https://doi.org/10.4049/jimmunol.178.12.7747
Huang, Julie H. ; Cárdenas-Navia, L. Isabel ; Caldwell, Charles C. ; Plumb, Troy J ; Radu, Caius G. ; Rocha, Paulo N. ; Wilder, Tuere ; Bromberg, Jonathan S. ; Cronstein, Bruce N. ; Sitkovsky, Michail ; Dewhirst, Mark W. ; Dustin, Michael L. / Requirements for T lymphocyte migration in explanted lymph nodes. In: Journal of Immunology. 2007 ; Vol. 178, No. 12. pp. 7747-7755.
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