Requirement of different signaling pathways mediated by insulin-like growth factor-I receptor for proliferation, invasion, and VPF/VEGF expression in a pancreatic carcinoma cell line

Huiyan Zeng, Kaustubh Datta, Matthias Neid, Jinping Li, Sareh Parangi, Debabrata Mukhopadhyay

Research output: Contribution to journalArticle

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Abstract

Several oncogenes and growth factors are found to be mutated and overexpressed in adenocarcinoma of the pancreas, and may correlate with its highly aggressive nature. Insulin-like growth factor (IGF-I) and its receptor (IGF-IR) are highly expressed in this tumor type. We examined the IGF-IR-mediated signaling pathways in relation to cell proliferation, invasiveness, and expression pattern of vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) in the pancreatic cancer line ASPC-1. Our findings show that IGF-IR is an important growth factor receptor for cell proliferation and invasion, and VPF/VEGF expression in ASPC-1. Further experiments indicate that IGF-IR mediates different signaling pathways to execute its functions. Activation of Ras by IGF-IR was found to be required for the cell invasion. On the other hand Src activation through IGF-IR is required for the cell proliferation, invasion, and also VPF/VEGF expression. Taken together, our data indicate the importance of IGF-IR in growth and invasiveness of the pancreatic cancer cell lines and also point out the multiple signaling pathways channeled through this receptor.

Original languageEnglish (US)
Pages (from-to)46-55
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume302
Issue number1
DOIs
StatePublished - Feb 28 2003

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IGF Type 1 Receptor
Vascular Endothelial Growth Factor A
Cells
Cell Line
Cell proliferation
Cell Proliferation
Pancreatic Neoplasms
Chemical activation
Growth Factor Receptors
Somatomedins
Pancreatic Carcinoma
Insulin-Like Growth Factor I
Oncogenes
Tumors
Pancreas
Intercellular Signaling Peptides and Proteins
Adenocarcinoma
Growth

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Requirement of different signaling pathways mediated by insulin-like growth factor-I receptor for proliferation, invasion, and VPF/VEGF expression in a pancreatic carcinoma cell line. / Zeng, Huiyan; Datta, Kaustubh; Neid, Matthias; Li, Jinping; Parangi, Sareh; Mukhopadhyay, Debabrata.

In: Biochemical and Biophysical Research Communications, Vol. 302, No. 1, 28.02.2003, p. 46-55.

Research output: Contribution to journalArticle

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AU - Mukhopadhyay, Debabrata

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