Replication protein A subunit 3 and the iron efficiency response in soybean

Sarah E. Atwood, Jamie A. O'Rourke, Gregory A. Peiffer, Tengfei Yin, Mahbubul Majumder, Chunquan Zhang, Silvia R. Cianzio, John H. Hill, Dianne Cook, Steven A. Whitham, Randy C. Shoemaker, Michelle A. Graham

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In soybean [Glycine max (L.) Merr.], iron deficiency results in interveinal chlorosis and decreased photosynthetic capacity, leading to stunting and yield loss. In this study, gene expression analyses investigated the role of soybean replication protein A (RPA) subunits during iron stress. Nine RPA homologs were significantly differentially expressed in response to iron stress in the near isogenic lines (NILs) Clark (iron efficient) and Isoclark (iron inefficient). RPA homologs exhibited opposing expression patterns in the two NILs, with RPA expression significantly repressed during iron deficiency in Clark but induced in Isoclark. We used virus induced gene silencing (VIGS) to repress GmRPA3 expression in the iron inefficient line Isoclark and mirror expression in Clark. GmRPA3-silenced plants had improved IDC symptoms and chlorophyll content under iron deficient conditions and also displayed stunted growth regardless of iron availability. RNA-Seq comparing gene expression between GmRPA3-silenced and empty vector plants revealed massive transcriptional reprogramming with differential expression of genes associated with defense, immunity, aging, death, protein modification, protein synthesis, photosynthesis and iron uptake and transport genes. Our findings suggest the iron efficient genotype Clark is able to induce energy controlling pathways, possibly regulated by SnRK1/TOR, to promote nutrient recycling and stress responses in iron deficient conditions.

Original languageEnglish (US)
Pages (from-to)213-234
Number of pages22
JournalPlant, Cell and Environment
Volume37
Issue number1
DOIs
StatePublished - Jan 2014

Fingerprint

Replication Protein A
Protein Subunits
Soybeans
Iron
iron
soybeans
proteins
Growth Disorders
isogenic lines
Gene Expression
gene expression
Hypochromic Anemia
Soybean Proteins
Photosynthesis
Gene Silencing
gene silencing
Chlorophyll
chlorosis
growth retardation
signs and symptoms (plants)

Keywords

  • Iron
  • Virus induced gene silencing

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Atwood, S. E., O'Rourke, J. A., Peiffer, G. A., Yin, T., Majumder, M., Zhang, C., ... Graham, M. A. (2014). Replication protein A subunit 3 and the iron efficiency response in soybean. Plant, Cell and Environment, 37(1), 213-234. https://doi.org/10.1111/pce.12147

Replication protein A subunit 3 and the iron efficiency response in soybean. / Atwood, Sarah E.; O'Rourke, Jamie A.; Peiffer, Gregory A.; Yin, Tengfei; Majumder, Mahbubul; Zhang, Chunquan; Cianzio, Silvia R.; Hill, John H.; Cook, Dianne; Whitham, Steven A.; Shoemaker, Randy C.; Graham, Michelle A.

In: Plant, Cell and Environment, Vol. 37, No. 1, 01.2014, p. 213-234.

Research output: Contribution to journalArticle

Atwood, SE, O'Rourke, JA, Peiffer, GA, Yin, T, Majumder, M, Zhang, C, Cianzio, SR, Hill, JH, Cook, D, Whitham, SA, Shoemaker, RC & Graham, MA 2014, 'Replication protein A subunit 3 and the iron efficiency response in soybean', Plant, Cell and Environment, vol. 37, no. 1, pp. 213-234. https://doi.org/10.1111/pce.12147
Atwood, Sarah E. ; O'Rourke, Jamie A. ; Peiffer, Gregory A. ; Yin, Tengfei ; Majumder, Mahbubul ; Zhang, Chunquan ; Cianzio, Silvia R. ; Hill, John H. ; Cook, Dianne ; Whitham, Steven A. ; Shoemaker, Randy C. ; Graham, Michelle A. / Replication protein A subunit 3 and the iron efficiency response in soybean. In: Plant, Cell and Environment. 2014 ; Vol. 37, No. 1. pp. 213-234.
@article{7fc6817890c84454822539634fa5a9d8,
title = "Replication protein A subunit 3 and the iron efficiency response in soybean",
abstract = "In soybean [Glycine max (L.) Merr.], iron deficiency results in interveinal chlorosis and decreased photosynthetic capacity, leading to stunting and yield loss. In this study, gene expression analyses investigated the role of soybean replication protein A (RPA) subunits during iron stress. Nine RPA homologs were significantly differentially expressed in response to iron stress in the near isogenic lines (NILs) Clark (iron efficient) and Isoclark (iron inefficient). RPA homologs exhibited opposing expression patterns in the two NILs, with RPA expression significantly repressed during iron deficiency in Clark but induced in Isoclark. We used virus induced gene silencing (VIGS) to repress GmRPA3 expression in the iron inefficient line Isoclark and mirror expression in Clark. GmRPA3-silenced plants had improved IDC symptoms and chlorophyll content under iron deficient conditions and also displayed stunted growth regardless of iron availability. RNA-Seq comparing gene expression between GmRPA3-silenced and empty vector plants revealed massive transcriptional reprogramming with differential expression of genes associated with defense, immunity, aging, death, protein modification, protein synthesis, photosynthesis and iron uptake and transport genes. Our findings suggest the iron efficient genotype Clark is able to induce energy controlling pathways, possibly regulated by SnRK1/TOR, to promote nutrient recycling and stress responses in iron deficient conditions.",
keywords = "Iron, Virus induced gene silencing",
author = "Atwood, {Sarah E.} and O'Rourke, {Jamie A.} and Peiffer, {Gregory A.} and Tengfei Yin and Mahbubul Majumder and Chunquan Zhang and Cianzio, {Silvia R.} and Hill, {John H.} and Dianne Cook and Whitham, {Steven A.} and Shoemaker, {Randy C.} and Graham, {Michelle A.}",
year = "2014",
month = "1",
doi = "10.1111/pce.12147",
language = "English (US)",
volume = "37",
pages = "213--234",
journal = "Plant, Cell and Environment",
issn = "0140-7791",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Replication protein A subunit 3 and the iron efficiency response in soybean

AU - Atwood, Sarah E.

AU - O'Rourke, Jamie A.

AU - Peiffer, Gregory A.

AU - Yin, Tengfei

AU - Majumder, Mahbubul

AU - Zhang, Chunquan

AU - Cianzio, Silvia R.

AU - Hill, John H.

AU - Cook, Dianne

AU - Whitham, Steven A.

AU - Shoemaker, Randy C.

AU - Graham, Michelle A.

PY - 2014/1

Y1 - 2014/1

N2 - In soybean [Glycine max (L.) Merr.], iron deficiency results in interveinal chlorosis and decreased photosynthetic capacity, leading to stunting and yield loss. In this study, gene expression analyses investigated the role of soybean replication protein A (RPA) subunits during iron stress. Nine RPA homologs were significantly differentially expressed in response to iron stress in the near isogenic lines (NILs) Clark (iron efficient) and Isoclark (iron inefficient). RPA homologs exhibited opposing expression patterns in the two NILs, with RPA expression significantly repressed during iron deficiency in Clark but induced in Isoclark. We used virus induced gene silencing (VIGS) to repress GmRPA3 expression in the iron inefficient line Isoclark and mirror expression in Clark. GmRPA3-silenced plants had improved IDC symptoms and chlorophyll content under iron deficient conditions and also displayed stunted growth regardless of iron availability. RNA-Seq comparing gene expression between GmRPA3-silenced and empty vector plants revealed massive transcriptional reprogramming with differential expression of genes associated with defense, immunity, aging, death, protein modification, protein synthesis, photosynthesis and iron uptake and transport genes. Our findings suggest the iron efficient genotype Clark is able to induce energy controlling pathways, possibly regulated by SnRK1/TOR, to promote nutrient recycling and stress responses in iron deficient conditions.

AB - In soybean [Glycine max (L.) Merr.], iron deficiency results in interveinal chlorosis and decreased photosynthetic capacity, leading to stunting and yield loss. In this study, gene expression analyses investigated the role of soybean replication protein A (RPA) subunits during iron stress. Nine RPA homologs were significantly differentially expressed in response to iron stress in the near isogenic lines (NILs) Clark (iron efficient) and Isoclark (iron inefficient). RPA homologs exhibited opposing expression patterns in the two NILs, with RPA expression significantly repressed during iron deficiency in Clark but induced in Isoclark. We used virus induced gene silencing (VIGS) to repress GmRPA3 expression in the iron inefficient line Isoclark and mirror expression in Clark. GmRPA3-silenced plants had improved IDC symptoms and chlorophyll content under iron deficient conditions and also displayed stunted growth regardless of iron availability. RNA-Seq comparing gene expression between GmRPA3-silenced and empty vector plants revealed massive transcriptional reprogramming with differential expression of genes associated with defense, immunity, aging, death, protein modification, protein synthesis, photosynthesis and iron uptake and transport genes. Our findings suggest the iron efficient genotype Clark is able to induce energy controlling pathways, possibly regulated by SnRK1/TOR, to promote nutrient recycling and stress responses in iron deficient conditions.

KW - Iron

KW - Virus induced gene silencing

UR - http://www.scopus.com/inward/record.url?scp=84897031883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897031883&partnerID=8YFLogxK

U2 - 10.1111/pce.12147

DO - 10.1111/pce.12147

M3 - Article

C2 - 23742135

AN - SCOPUS:84897031883

VL - 37

SP - 213

EP - 234

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

SN - 0140-7791

IS - 1

ER -