Replication of porcine parvovirus in peripheral blood lymphocytes, monocytes, and peritoneal macrophages

P. S. Paul, W. L. Mengeling, T. T. Brown

Research output: Contribution to journalArticle

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Abstract

Porcine peripheral blood lymphocytes (PBL), peripheral blood monocytes, and peritoneal macrophages were examined for their ability to support porcine parvovirus (PPV) replication. The cell cultures were infected with the NADL-2 strain of PPV at 0.1 multiplicity of infection. PBL cultures were stimulated with the following phytomitogens: phytohemagglutinin M, concanavalin A, and pokeweed mitogen. Unstimulated PBL cultures infected with PPV and uninfected PBL stimulated with phytomitogens served as controls. All cultures were examined daily for PPV-specific immunofluorescence and hemagglutinin. PPV replicated in PBL cultures stimulated with all phytomitogens. Both viral hemagglutinin in culture fluids and nuclear immunofluorerscence in cells were detected. In contrast, unstimulated PBL did not support viral replication; however, PPV antigen was detected in the cytoplasm. PPV persisted in unstimulated PBL for 21 days (duration of the experiment) without replication, but replicated each time with the addition of phytohemagglutinin M at 0, 3, 7, 14, and 21 days after infection. Uninfected PBL stimulated with phytomitogens lacked both viral hemagglutinin and immunofluorescence. Simultaneous detection of lymphocyte surface marker and viral antigens in pokeweed mitogen-stimulated PBL revealed that both T and non-T cells (B and null cells) are able to support PPV replication. Peripheral blood monocytes and peritoneal macrophages phagocytized PPV but did not support virus replication.

Original languageEnglish (US)
Pages (from-to)1003-1007
Number of pages5
JournalInfection and immunity
Volume25
Issue number3
StatePublished - Dec 1 1979

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Porcine Parvovirus
Peritoneal Macrophages
Monocytes
Lymphocytes
Mitogens
Viral Hemagglutinins
Pokeweed Mitogens
Fluorescent Antibody Technique
Null Lymphocytes
Viral Antigens
Differentiation Antigens
Hemagglutinins
Surface Antigens
Virus Replication
Concanavalin A
Infection

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Replication of porcine parvovirus in peripheral blood lymphocytes, monocytes, and peritoneal macrophages. / Paul, P. S.; Mengeling, W. L.; Brown, T. T.

In: Infection and immunity, Vol. 25, No. 3, 01.12.1979, p. 1003-1007.

Research output: Contribution to journalArticle

Paul, P. S. ; Mengeling, W. L. ; Brown, T. T. / Replication of porcine parvovirus in peripheral blood lymphocytes, monocytes, and peritoneal macrophages. In: Infection and immunity. 1979 ; Vol. 25, No. 3. pp. 1003-1007.
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