Replication of coxsackievirus B3 in primary cell cultures generates novel viral genome deletions

K. S. Kim, N. M. Chapman, S. Tracy

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Coxsackievirus B3 (CVB3) generates 5′-terminally deleted genomes (TDs) during replication in murine hearts. We show here that CVB3 populations with TDs can also be generated within two to three passages of CVB3 in primary, but not immortalized, cell cultures. Deletions of less than 49 nucleotides increase in size during passage, while 5′ TDs of 49 nucleotides appear to be the maximum deletion size. The cellular environment of contact-inhibited primary cell cultures or the myocardium in vivo is sufficient for the selection of 5′ TDs over undeleted genomes.

Original languageEnglish (US)
Pages (from-to)2033-2037
Number of pages5
JournalJournal of virology
Volume82
Issue number4
DOIs
StatePublished - Feb 1 2008

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Enterovirus
Primary Cell Culture
Viral Genome
cell culture
genome
Nucleotides
nucleotides
primary contact
Genome
myocardium
Myocardium
Cell Culture Techniques
heart
mice
Population

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Replication of coxsackievirus B3 in primary cell cultures generates novel viral genome deletions. / Kim, K. S.; Chapman, N. M.; Tracy, S.

In: Journal of virology, Vol. 82, No. 4, 01.02.2008, p. 2033-2037.

Research output: Contribution to journalArticle

Kim, K. S. ; Chapman, N. M. ; Tracy, S. / Replication of coxsackievirus B3 in primary cell cultures generates novel viral genome deletions. In: Journal of virology. 2008 ; Vol. 82, No. 4. pp. 2033-2037.
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