Repeated elevational transitions in hemoglobin function during the evolution of Andean hummingbirds

Joana Projecto-Garcia, Chandrasekhar Natarajan, Hideaki Moriyama, Roy E. Weber, Angela Fago, Zachary A. Cheviron, Robert Dudley, Jimmy A. McGuire, Christopher C. Witt, Jay F Storz

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Animals that sustain high levels of aerobic activity under hypoxic conditions (e.g., birds that fly at high altitude) face the physiological challenge of jointly optimizing blood-O2 affinity for O2 loading in the pulmonary circulation and O2 unloading in the systemic circulation. At high altitude, this challenge is especially acute for small endotherms like hummingbirds that have exceedingly high mass-specific metabolic rates. Here we report an experimental analysis of hemoglobin (Hb) function in South American hummingbirds that revealed a positive correlation between Hb-O2 affinity and native elevation. Protein engineering experiments and ancestral- state reconstructions revealed that this correlation is attributable to derived increases in Hb-O2 affinity in highland lineages, as well as derived reductions in Hb-O2 affinity in lowland lineages. Site-directed mutagenesis experiments demonstrated that repeated evolutionary transitions in biochemical phenotype are mainly attributable to repeated amino acid replacements at two epistatically interacting sites that alter the allosteric regulation of Hb-O2 affinity. These results demonstrate that repeated changes in biochemical phenotype involve parallelism at the molecular level, and that mutations with indirect, second-order effects on Hb allostery play key roles in biochemical adaptation.

Original languageEnglish (US)
Pages (from-to)20669-20674
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number51
DOIs
StatePublished - Dec 17 2013

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hummingbirds
hemoglobin
protein engineering
phenotype
site-directed mutagenesis
anaerobic conditions
lowlands
highlands
lungs
mutation
amino acids
birds
blood
animals

Keywords

  • Epistasis
  • High-altitude adaptation
  • Hypoxia
  • Parallel evolution
  • Protein evolution

ASJC Scopus subject areas

  • General

Cite this

Repeated elevational transitions in hemoglobin function during the evolution of Andean hummingbirds. / Projecto-Garcia, Joana; Natarajan, Chandrasekhar; Moriyama, Hideaki; Weber, Roy E.; Fago, Angela; Cheviron, Zachary A.; Dudley, Robert; McGuire, Jimmy A.; Witt, Christopher C.; Storz, Jay F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 51, 17.12.2013, p. 20669-20674.

Research output: Contribution to journalArticle

Projecto-Garcia, Joana ; Natarajan, Chandrasekhar ; Moriyama, Hideaki ; Weber, Roy E. ; Fago, Angela ; Cheviron, Zachary A. ; Dudley, Robert ; McGuire, Jimmy A. ; Witt, Christopher C. ; Storz, Jay F. / Repeated elevational transitions in hemoglobin function during the evolution of Andean hummingbirds. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 51. pp. 20669-20674.
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