Repair of Short Oligodeoxyribonucleotides Containing A Stable Adduct and An Apurinic Site by Extracts of MCF-10A1 Cells

Kimberly A. Chapman, Ercole Cavalieri, Eleanor G Rogan

Research output: Contribution to journalArticle

Abstract

Background: DNA with two sites of damage in close proximity might not be repaired as efficiently as DNA with a single damage site. Materials and Methods: To study this hypothesis, we utilized short oligodeoxyribonucleotides with a stable adduct 7 or 16 nucleotides (nt) downstream from an apurinic (AP) site. Repair by extracts of human breast epithelial MCF-10A1 cells was assayed by quantifying the incorporation of [α-32P]dTTP. Results: The level of repair of an oligodeoxyribonucleotide with an AP site 7 nt from a stable adduct was comparable to that of the oligodeoxyribonucleotide with only an AP site. A decrease in overall repair of oligodeoxyribonucleotides containing an AP site and a stable adduct was observed if these lesions were 16 nt apart compared to the presence of only an AP site. Conclusion: The ability of human breast MCF-10A1 cells to repair DNA adducts and AP sites is affected by other near-by lesions.

Original languageEnglish (US)
Pages (from-to)117-121
Number of pages5
JournalAnticancer Research
Volume24
Issue number1
StatePublished - Jan 1 2004

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Oligodeoxyribonucleotides
Nucleotides
Breast
DNA Adducts
DNA

Keywords

  • Apurinic sites
  • Base excision repair
  • DNA adducts
  • DNA repair
  • Human breast cell extracts
  • Nucleotide excision repair

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Repair of Short Oligodeoxyribonucleotides Containing A Stable Adduct and An Apurinic Site by Extracts of MCF-10A1 Cells. / Chapman, Kimberly A.; Cavalieri, Ercole; Rogan, Eleanor G.

In: Anticancer Research, Vol. 24, No. 1, 01.01.2004, p. 117-121.

Research output: Contribution to journalArticle

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abstract = "Background: DNA with two sites of damage in close proximity might not be repaired as efficiently as DNA with a single damage site. Materials and Methods: To study this hypothesis, we utilized short oligodeoxyribonucleotides with a stable adduct 7 or 16 nucleotides (nt) downstream from an apurinic (AP) site. Repair by extracts of human breast epithelial MCF-10A1 cells was assayed by quantifying the incorporation of [α-32P]dTTP. Results: The level of repair of an oligodeoxyribonucleotide with an AP site 7 nt from a stable adduct was comparable to that of the oligodeoxyribonucleotide with only an AP site. A decrease in overall repair of oligodeoxyribonucleotides containing an AP site and a stable adduct was observed if these lesions were 16 nt apart compared to the presence of only an AP site. Conclusion: The ability of human breast MCF-10A1 cells to repair DNA adducts and AP sites is affected by other near-by lesions.",
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AU - Rogan, Eleanor G

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N2 - Background: DNA with two sites of damage in close proximity might not be repaired as efficiently as DNA with a single damage site. Materials and Methods: To study this hypothesis, we utilized short oligodeoxyribonucleotides with a stable adduct 7 or 16 nucleotides (nt) downstream from an apurinic (AP) site. Repair by extracts of human breast epithelial MCF-10A1 cells was assayed by quantifying the incorporation of [α-32P]dTTP. Results: The level of repair of an oligodeoxyribonucleotide with an AP site 7 nt from a stable adduct was comparable to that of the oligodeoxyribonucleotide with only an AP site. A decrease in overall repair of oligodeoxyribonucleotides containing an AP site and a stable adduct was observed if these lesions were 16 nt apart compared to the presence of only an AP site. Conclusion: The ability of human breast MCF-10A1 cells to repair DNA adducts and AP sites is affected by other near-by lesions.

AB - Background: DNA with two sites of damage in close proximity might not be repaired as efficiently as DNA with a single damage site. Materials and Methods: To study this hypothesis, we utilized short oligodeoxyribonucleotides with a stable adduct 7 or 16 nucleotides (nt) downstream from an apurinic (AP) site. Repair by extracts of human breast epithelial MCF-10A1 cells was assayed by quantifying the incorporation of [α-32P]dTTP. Results: The level of repair of an oligodeoxyribonucleotide with an AP site 7 nt from a stable adduct was comparable to that of the oligodeoxyribonucleotide with only an AP site. A decrease in overall repair of oligodeoxyribonucleotides containing an AP site and a stable adduct was observed if these lesions were 16 nt apart compared to the presence of only an AP site. Conclusion: The ability of human breast MCF-10A1 cells to repair DNA adducts and AP sites is affected by other near-by lesions.

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KW - Base excision repair

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