Renal tubular absorption of D-glucose, 3-O-methyl-D-glucose, and 2-deoxy-D-glucose

T. Knight, Steven Claude Sansom, E. J. Weinman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Intratubular microinjection studies were performed in the rat to determine the nephron sites of reabsorption of D-glucose, 3-O-methyl-D-glucose, and 2-deoxy-D-glucose. Urinary recoveries of D-glucose averaged 4±1% from early proximal tubule sites (EPT), 27±5% from late proximal tubule sites (LPT), and 99±2% following microinjections into the distal convoluted tubule (DCT). 3-O-Methyl-D-glucose recoveries were 81±2, 89±2, and 101±5% from EPT, LPT, and DCT, respectively. 2-Deoxy-D-glucose recoveries were 77±3 from EPT, 87±3 from LPT, and 91±1% from DCT. The addition of phloridzin to the microinjection solution significantly increased the recoveries of D-glucose (84±2%) and 3-O-methyl-D-glucose (102±2%) from EPT. Recoveries of 2-deoxy-D-glucose with phloridzin averaged 92±1,93±2, and 100±2% from EPT, LPT, and DCT sites. These studies indicate that all three of the sugars studied are reabsorbed in the proximal tubule and that 2-deoxy-D-glucose has an additional site of reabsorption at a nephron site beyond the distal tubule. Phloridzin inhibits the reabsorption of these sugars, suggesting that their reabsorption is mediated by a carrier mechanism. Since prior studies from this laboratory have indicated that D-glucose and 3-O-methyl-D-glucose, but 2-deoxy-D-glucose, 2-deoxy-d-glucose, enhance sodium and water reabsorption in the proximal tubule, the present studies may indicate either that the enhancement of water reabsorption by some hexose sugars is not simply the consequence of the stimulation of a hexose-sodium transport system or that the reabsorption of some hexose sugars is not sodium dependent.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume2
Issue number4
StatePublished - Jan 1 1977

Fingerprint

3-O-Methylglucose
Deoxyglucose
Phlorhizin
Hexoses
Microinjections
Glucose
Sodium
Nephrons
Water
Renal Reabsorption

ASJC Scopus subject areas

  • Physiology

Cite this

@article{1cbeb09bb9a144b98332bbb8cc440f38,
title = "Renal tubular absorption of D-glucose, 3-O-methyl-D-glucose, and 2-deoxy-D-glucose",
abstract = "Intratubular microinjection studies were performed in the rat to determine the nephron sites of reabsorption of D-glucose, 3-O-methyl-D-glucose, and 2-deoxy-D-glucose. Urinary recoveries of D-glucose averaged 4±1{\%} from early proximal tubule sites (EPT), 27±5{\%} from late proximal tubule sites (LPT), and 99±2{\%} following microinjections into the distal convoluted tubule (DCT). 3-O-Methyl-D-glucose recoveries were 81±2, 89±2, and 101±5{\%} from EPT, LPT, and DCT, respectively. 2-Deoxy-D-glucose recoveries were 77±3 from EPT, 87±3 from LPT, and 91±1{\%} from DCT. The addition of phloridzin to the microinjection solution significantly increased the recoveries of D-glucose (84±2{\%}) and 3-O-methyl-D-glucose (102±2{\%}) from EPT. Recoveries of 2-deoxy-D-glucose with phloridzin averaged 92±1,93±2, and 100±2{\%} from EPT, LPT, and DCT sites. These studies indicate that all three of the sugars studied are reabsorbed in the proximal tubule and that 2-deoxy-D-glucose has an additional site of reabsorption at a nephron site beyond the distal tubule. Phloridzin inhibits the reabsorption of these sugars, suggesting that their reabsorption is mediated by a carrier mechanism. Since prior studies from this laboratory have indicated that D-glucose and 3-O-methyl-D-glucose, but 2-deoxy-D-glucose, 2-deoxy-d-glucose, enhance sodium and water reabsorption in the proximal tubule, the present studies may indicate either that the enhancement of water reabsorption by some hexose sugars is not simply the consequence of the stimulation of a hexose-sodium transport system or that the reabsorption of some hexose sugars is not sodium dependent.",
author = "T. Knight and Sansom, {Steven Claude} and Weinman, {E. J.}",
year = "1977",
month = "1",
day = "1",
language = "English (US)",
volume = "2",
journal = "American Journal of Physiology - Renal Physiology",
issn = "0363-6127",
publisher = "American Physiological Society",
number = "4",

}

TY - JOUR

T1 - Renal tubular absorption of D-glucose, 3-O-methyl-D-glucose, and 2-deoxy-D-glucose

AU - Knight, T.

AU - Sansom, Steven Claude

AU - Weinman, E. J.

PY - 1977/1/1

Y1 - 1977/1/1

N2 - Intratubular microinjection studies were performed in the rat to determine the nephron sites of reabsorption of D-glucose, 3-O-methyl-D-glucose, and 2-deoxy-D-glucose. Urinary recoveries of D-glucose averaged 4±1% from early proximal tubule sites (EPT), 27±5% from late proximal tubule sites (LPT), and 99±2% following microinjections into the distal convoluted tubule (DCT). 3-O-Methyl-D-glucose recoveries were 81±2, 89±2, and 101±5% from EPT, LPT, and DCT, respectively. 2-Deoxy-D-glucose recoveries were 77±3 from EPT, 87±3 from LPT, and 91±1% from DCT. The addition of phloridzin to the microinjection solution significantly increased the recoveries of D-glucose (84±2%) and 3-O-methyl-D-glucose (102±2%) from EPT. Recoveries of 2-deoxy-D-glucose with phloridzin averaged 92±1,93±2, and 100±2% from EPT, LPT, and DCT sites. These studies indicate that all three of the sugars studied are reabsorbed in the proximal tubule and that 2-deoxy-D-glucose has an additional site of reabsorption at a nephron site beyond the distal tubule. Phloridzin inhibits the reabsorption of these sugars, suggesting that their reabsorption is mediated by a carrier mechanism. Since prior studies from this laboratory have indicated that D-glucose and 3-O-methyl-D-glucose, but 2-deoxy-D-glucose, 2-deoxy-d-glucose, enhance sodium and water reabsorption in the proximal tubule, the present studies may indicate either that the enhancement of water reabsorption by some hexose sugars is not simply the consequence of the stimulation of a hexose-sodium transport system or that the reabsorption of some hexose sugars is not sodium dependent.

AB - Intratubular microinjection studies were performed in the rat to determine the nephron sites of reabsorption of D-glucose, 3-O-methyl-D-glucose, and 2-deoxy-D-glucose. Urinary recoveries of D-glucose averaged 4±1% from early proximal tubule sites (EPT), 27±5% from late proximal tubule sites (LPT), and 99±2% following microinjections into the distal convoluted tubule (DCT). 3-O-Methyl-D-glucose recoveries were 81±2, 89±2, and 101±5% from EPT, LPT, and DCT, respectively. 2-Deoxy-D-glucose recoveries were 77±3 from EPT, 87±3 from LPT, and 91±1% from DCT. The addition of phloridzin to the microinjection solution significantly increased the recoveries of D-glucose (84±2%) and 3-O-methyl-D-glucose (102±2%) from EPT. Recoveries of 2-deoxy-D-glucose with phloridzin averaged 92±1,93±2, and 100±2% from EPT, LPT, and DCT sites. These studies indicate that all three of the sugars studied are reabsorbed in the proximal tubule and that 2-deoxy-D-glucose has an additional site of reabsorption at a nephron site beyond the distal tubule. Phloridzin inhibits the reabsorption of these sugars, suggesting that their reabsorption is mediated by a carrier mechanism. Since prior studies from this laboratory have indicated that D-glucose and 3-O-methyl-D-glucose, but 2-deoxy-D-glucose, 2-deoxy-d-glucose, enhance sodium and water reabsorption in the proximal tubule, the present studies may indicate either that the enhancement of water reabsorption by some hexose sugars is not simply the consequence of the stimulation of a hexose-sodium transport system or that the reabsorption of some hexose sugars is not sodium dependent.

UR - http://www.scopus.com/inward/record.url?scp=0017746732&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017746732&partnerID=8YFLogxK

M3 - Article

VL - 2

JO - American Journal of Physiology - Renal Physiology

JF - American Journal of Physiology - Renal Physiology

SN - 0363-6127

IS - 4

ER -