Renal fluid and electrolyte handling in BKCa-β1-/- mice

Jennifer L. Pluznick, Peilin Wei, Pamela K Carmines, Steven Claude Sansom

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Large-conductance Ca2+-activated K+ channels (BKCa) are composed of pore-forming α-subunits and one of four accessory β-subunits. The β1subunit, found predominantly in smooth muscle, modulates the Ca2+ sensitivity and pharmacological properties of BKCa. BKCa-β1 null mice (Mβ1-/-) are moderately hypertensive, consistent with the role of BKCa in modulating intrinsic vascular tone. Because BKCa are present in various renal cells including the mesangium and cortical collecting ducts, we determined whether fluid or electrolyte excretion was impaired in Mβ1-/- under euvolemic, volume-expanded, or high-salt diet conditions. Under euvolemic conditions, no differences in renal function were found between Mβ1-/- and Mβ1+/+. However, glomerular filtration rate (GFR) and fractional K+ excretion were significantly impaired in Mβ1-/- in response to acute volume expansion. In contrast, Mβ1-/- exhibited enhanced Na+ excretion and fractional Na+ excretion responses to acute volume expansion. Differences in renal function between Mβ1+/+ and Mβ1-/- were not observed when chronically treated with a high-salt diet. These observations indicate that the β1-subunit of BKCa contributes to the increased GFR that accompanies an acute salt and volume load and raises the possibility that it is also involved in regulating K+ excretion under these conditions.

Original languageEnglish (US)
Pages (from-to)F1274-F1279
JournalAmerican Journal of Physiology - Renal Physiology
Volume284
Issue number6 53-6
StatePublished - Jun 1 2003

Fingerprint

Electrolytes
Salts
Glomerular Filtration Rate
Kidney
Diet
Calcium-Activated Potassium Channels
Smooth Muscle
Blood Vessels
Pharmacology

Keywords

  • Glomerular filtration rate
  • Large-conductance, calcium-activated potassium channels
  • Maxi K channel
  • Potassium excretion
  • Volume expansion

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Renal fluid and electrolyte handling in BKCa-β1-/- mice. / Pluznick, Jennifer L.; Wei, Peilin; Carmines, Pamela K; Sansom, Steven Claude.

In: American Journal of Physiology - Renal Physiology, Vol. 284, No. 6 53-6, 01.06.2003, p. F1274-F1279.

Research output: Contribution to journalArticle

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