Renal denervation improves sodium excretion in rats with chronic heart failure: effects on expression of renal ENaC and AQP2

Hong Zheng, Xuefei Liu, Kenichi Katsurada, Kaushik P. Patel

Research output: Contribution to journalArticle

Abstract

Previously we have shown that increased expression of renal epithelial sodium channels (ENaC) may contribute to the renal sodium and water retention observed during chronic heart failure (CHF). The goal of this study was to examine whether renal denervation (RDN) changed the expressions of renal sodium transporters ENaC, sodium-hydrogen exchanger-3 proteins (NHE3), and water channel aquaporin 2 (AQP2) in rats with CHF. CHF was produced by left coronary artery ligation in rats. Four weeks after ligation surgery, surgical bilateral RDN was performed. The expression of ENaC, NHE3, and AQP2 in both renal cortex and medulla were measured. As a functional test for ENaC activation, diuretic and natriuretic responses to ENaC inhibitor benzamil were monitored in four groups of rats (Sham, Sham+RDN, CHF, CHF+RDN). Western blot analysis indicated that RDN (1 wk later) significantly reduced protein levels of α-ENaC, β-ENaC, γ-ENaC, and AQP2 in the renal cortex of CHF rats. RDN had no significant effects on the protein expression of kidney NHE3 in both Sham and CHF rats. Immunofluorescence studies of kidney sections confirmed the reduced signaling of ENaC and AQP2 in the CHF+RDN rats compared with the CHF rats. There were increases in diuretic and natriuretic responses to ENaC inhibitor benzamil in rats with CHF. RDN reduced the diuretic and natriuretic responses to benzamil in CHF rats. These findings suggest a critical role for renal nerves in the enhanced expression of ENaC and AQP2 and subsequent pathophysiology of renal sodium and water retention associated with CHF.NEW & NOTEWORTHY This is the first study to show in a comprehensive way that renal denervation initiated after a period of chronic heart failure reduces the expression of epithelial sodium channels and aquaporin 2 leading to reduced epithelial sodium channel function and sodium retention.

Original languageEnglish (US)
Pages (from-to)H958-H968
JournalAmerican journal of physiology. Heart and circulatory physiology
Volume317
Issue number5
DOIs
StatePublished - Nov 1 2019

Fingerprint

Aquaporin 2
Denervation
Heart Failure
Sodium
Kidney
Epithelial Sodium Channels
Diuretics
Chronic Kidney Failure
Ligation
Aquaporins

Keywords

  • heart failure
  • renal function
  • renal nerve
  • sympathetic nerve activity

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Renal denervation improves sodium excretion in rats with chronic heart failure : effects on expression of renal ENaC and AQP2. / Zheng, Hong; Liu, Xuefei; Katsurada, Kenichi; Patel, Kaushik P.

In: American journal of physiology. Heart and circulatory physiology, Vol. 317, No. 5, 01.11.2019, p. H958-H968.

Research output: Contribution to journalArticle

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