Renal cortical nitric oxide synthase activity during maturational growth in the rat

Naohito Ishii, Keiji Fujiwara, Pascale H. Lane, Kaushik P Patel, Pamela K Carmines

Research output: Contribution to journalReview article

6 Citations (Scopus)

Abstract

The present study was designed to test the hypothesis that growth from puberty to adulthood in the rat is associated with an increase in renal cortical nitric oxide synthase (NOS) activity that results in an augmented impact of nitric oxide (NO) on hemodynamic function. Two groups of male Sprague-Dawley rats were studied: juvenile rats (∼2 months old) and mature rats (∼5 months old). NOS activity, measured as Nω -nitro-L-arginine (NNA)-sensitive 3H-L-citrulline production from 3H-L-arginine, was significantly higher in the renal cortex of mature rats (57±2 pmol/h per mg protein) than in juveniles (42±3 pmol/h per mg protein). Additional animals from each group were anesthetized to determine the acute impact of NOS inhibition on arterial pressure and renal cortical blood flow, measured by single-fiber Doppler flowmetry. Cortical blood flow was higher in mature rats than in juveniles, averaging 22±2 and 16±1 perfusion units, respectively. NOS inhibition (10 mg/kg NNA i.v.) decreased renal cortical blood flow in mature rats by 35±7%, but only by 9±4% in juvenile animals. These data support the hypothesis that maturational growth in the rat is associated with augmented NOS activity coupled with an increased tonic influence of NO on renal cortical blood flow.

Original languageEnglish (US)
Pages (from-to)591-596
Number of pages6
JournalPediatric Nephrology
Volume17
Issue number8
DOIs
StatePublished - Dec 1 2002

Fingerprint

Nitric Oxide Synthase
Kidney
Growth
Renal Circulation
Arginine
Nitric Oxide
Citrulline
Rheology
Puberty
Sprague Dawley Rats
Arterial Pressure
Proteins
Perfusion
Hemodynamics

Keywords

  • Adult
  • Nitric oxide
  • Nitro-L-arginne
  • Puberty
  • Renal cortical blood flow

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Nephrology

Cite this

Renal cortical nitric oxide synthase activity during maturational growth in the rat. / Ishii, Naohito; Fujiwara, Keiji; Lane, Pascale H.; Patel, Kaushik P; Carmines, Pamela K.

In: Pediatric Nephrology, Vol. 17, No. 8, 01.12.2002, p. 591-596.

Research output: Contribution to journalReview article

Ishii, Naohito ; Fujiwara, Keiji ; Lane, Pascale H. ; Patel, Kaushik P ; Carmines, Pamela K. / Renal cortical nitric oxide synthase activity during maturational growth in the rat. In: Pediatric Nephrology. 2002 ; Vol. 17, No. 8. pp. 591-596.
@article{7d59966745d245c2bd7a6d016819d407,
title = "Renal cortical nitric oxide synthase activity during maturational growth in the rat",
abstract = "The present study was designed to test the hypothesis that growth from puberty to adulthood in the rat is associated with an increase in renal cortical nitric oxide synthase (NOS) activity that results in an augmented impact of nitric oxide (NO) on hemodynamic function. Two groups of male Sprague-Dawley rats were studied: juvenile rats (∼2 months old) and mature rats (∼5 months old). NOS activity, measured as Nω -nitro-L-arginine (NNA)-sensitive 3H-L-citrulline production from 3H-L-arginine, was significantly higher in the renal cortex of mature rats (57±2 pmol/h per mg protein) than in juveniles (42±3 pmol/h per mg protein). Additional animals from each group were anesthetized to determine the acute impact of NOS inhibition on arterial pressure and renal cortical blood flow, measured by single-fiber Doppler flowmetry. Cortical blood flow was higher in mature rats than in juveniles, averaging 22±2 and 16±1 perfusion units, respectively. NOS inhibition (10 mg/kg NNA i.v.) decreased renal cortical blood flow in mature rats by 35±7{\%}, but only by 9±4{\%} in juvenile animals. These data support the hypothesis that maturational growth in the rat is associated with augmented NOS activity coupled with an increased tonic influence of NO on renal cortical blood flow.",
keywords = "Adult, Nitric oxide, Nitro-L-arginne, Puberty, Renal cortical blood flow",
author = "Naohito Ishii and Keiji Fujiwara and Lane, {Pascale H.} and Patel, {Kaushik P} and Carmines, {Pamela K}",
year = "2002",
month = "12",
day = "1",
doi = "10.1007/s00467-002-0859-5",
language = "English (US)",
volume = "17",
pages = "591--596",
journal = "Pediatric Nephrology",
issn = "0931-041X",
publisher = "Springer Verlag",
number = "8",

}

TY - JOUR

T1 - Renal cortical nitric oxide synthase activity during maturational growth in the rat

AU - Ishii, Naohito

AU - Fujiwara, Keiji

AU - Lane, Pascale H.

AU - Patel, Kaushik P

AU - Carmines, Pamela K

PY - 2002/12/1

Y1 - 2002/12/1

N2 - The present study was designed to test the hypothesis that growth from puberty to adulthood in the rat is associated with an increase in renal cortical nitric oxide synthase (NOS) activity that results in an augmented impact of nitric oxide (NO) on hemodynamic function. Two groups of male Sprague-Dawley rats were studied: juvenile rats (∼2 months old) and mature rats (∼5 months old). NOS activity, measured as Nω -nitro-L-arginine (NNA)-sensitive 3H-L-citrulline production from 3H-L-arginine, was significantly higher in the renal cortex of mature rats (57±2 pmol/h per mg protein) than in juveniles (42±3 pmol/h per mg protein). Additional animals from each group were anesthetized to determine the acute impact of NOS inhibition on arterial pressure and renal cortical blood flow, measured by single-fiber Doppler flowmetry. Cortical blood flow was higher in mature rats than in juveniles, averaging 22±2 and 16±1 perfusion units, respectively. NOS inhibition (10 mg/kg NNA i.v.) decreased renal cortical blood flow in mature rats by 35±7%, but only by 9±4% in juvenile animals. These data support the hypothesis that maturational growth in the rat is associated with augmented NOS activity coupled with an increased tonic influence of NO on renal cortical blood flow.

AB - The present study was designed to test the hypothesis that growth from puberty to adulthood in the rat is associated with an increase in renal cortical nitric oxide synthase (NOS) activity that results in an augmented impact of nitric oxide (NO) on hemodynamic function. Two groups of male Sprague-Dawley rats were studied: juvenile rats (∼2 months old) and mature rats (∼5 months old). NOS activity, measured as Nω -nitro-L-arginine (NNA)-sensitive 3H-L-citrulline production from 3H-L-arginine, was significantly higher in the renal cortex of mature rats (57±2 pmol/h per mg protein) than in juveniles (42±3 pmol/h per mg protein). Additional animals from each group were anesthetized to determine the acute impact of NOS inhibition on arterial pressure and renal cortical blood flow, measured by single-fiber Doppler flowmetry. Cortical blood flow was higher in mature rats than in juveniles, averaging 22±2 and 16±1 perfusion units, respectively. NOS inhibition (10 mg/kg NNA i.v.) decreased renal cortical blood flow in mature rats by 35±7%, but only by 9±4% in juvenile animals. These data support the hypothesis that maturational growth in the rat is associated with augmented NOS activity coupled with an increased tonic influence of NO on renal cortical blood flow.

KW - Adult

KW - Nitric oxide

KW - Nitro-L-arginne

KW - Puberty

KW - Renal cortical blood flow

UR - http://www.scopus.com/inward/record.url?scp=0036952148&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036952148&partnerID=8YFLogxK

U2 - 10.1007/s00467-002-0859-5

DO - 10.1007/s00467-002-0859-5

M3 - Review article

C2 - 12185463

AN - SCOPUS:0036952148

VL - 17

SP - 591

EP - 596

JO - Pediatric Nephrology

JF - Pediatric Nephrology

SN - 0931-041X

IS - 8

ER -