Removal of reactive oxygen species-induced 3′-blocked ends by XPF-ERCC1

Laura A. Fisher, Laura Samson, Tadayoshi Bessho

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

XPF-ERCC1 is a structure-specific endonuclease that is essential for nucleotide excision repair and DNA interstrand cross-link repair in mammalian cells. The yeast counterpart of XPF-ERCC1, Rad1-Rad10, plays multiple roles in DNA repair. Rad1-Rad10 is implicated to be involved in the repair of oxidative DNA damage. To explore the role(s) of XPF-ERCC1 in the repair of DNA damage induced by reactive oxygen species (ROS), cellular sensitivity of the XPF-deficient Chinese hamster ovary cell line UV41 to ROS was investigated. The XPF-deficient UV41 showed sensitivity to hydrogen peroxide, bleomycin, and paraquat. Furthermore, XPF-ERCC1 showed an ability to remove 3′-blocked ends such as 3′-phosphoglycolate from the 3′-end of DNA in vitro. These data suggest that XPF-ERCC1 plays a role in the repair of ROS-induced DNA damage by trimming 3′-blocked ends. The accumulation of various types of DNA damage, including ROS-induced DNA damage due to defects in multiple XPF-ERCC1-mediated DNA repair pathways, could contribute to the accelerated aging phenotypes observed in an XPF-ERCC1-deficient patient.

Original languageEnglish (US)
Pages (from-to)1876-1881
Number of pages6
JournalChemical Research in Toxicology
Volume24
Issue number11
DOIs
StatePublished - Nov 21 2011

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DNA Damage
Reactive Oxygen Species
Repair
DNA Repair
DNA
Paraquat
Endonucleases
Bleomycin
Cricetulus
Hydrogen Peroxide
Ovary
Cells
Yeasts
Phenotype
Trimming
Cell Line
Yeast
Nucleotides
Aging of materials
Defects

ASJC Scopus subject areas

  • Toxicology

Cite this

Removal of reactive oxygen species-induced 3′-blocked ends by XPF-ERCC1. / Fisher, Laura A.; Samson, Laura; Bessho, Tadayoshi.

In: Chemical Research in Toxicology, Vol. 24, No. 11, 21.11.2011, p. 1876-1881.

Research output: Contribution to journalArticle

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