Removal of contaminating proteases from Bacillus thuringiensis parasporal crystals by density gradient centrifugation in NaBr

Kenneth Nickerson, Jane D. Swanson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Purification of the Bacillus thuringiensis protein crystal on NaBr density gradients confers a significant technical advantage in that the crystal-associated proteases are thereby removed. The use of protease-free crystals allows reliable determination of native crystal parameters.

Original languageEnglish (US)
Pages (from-to)213-215
Number of pages3
JournalEuropean Journal of Applied Microbiology and Biotechnology
Volume13
Issue number4
DOIs
StatePublished - Dec 1 1981

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Bacillus thuringiensis
Density Gradient Centrifugation
Peptide Hydrolases
Bacillus Thuringiensis insecticidal crystal protein

ASJC Scopus subject areas

  • Microbiology
  • Biotechnology
  • Medicine(all)

Cite this

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title = "Removal of contaminating proteases from Bacillus thuringiensis parasporal crystals by density gradient centrifugation in NaBr",
abstract = "Purification of the Bacillus thuringiensis protein crystal on NaBr density gradients confers a significant technical advantage in that the crystal-associated proteases are thereby removed. The use of protease-free crystals allows reliable determination of native crystal parameters.",
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AU - Swanson, Jane D.

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