Relief of mg 2+-dependent inhibition of trpm1 by pkcα at the rod bipolar cell synapse

Melissa Ann F Rampino, Scott A Nawy

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In the retina, light onset hyperpolarizes photoreceptors and depolarizes ON bipolar cells at the sign inverting photoreceptor-ON bipolar cell synapse. Transmission at this synapse is mediated by a signaling cascade comprised of mGluR6, a G-protein containing G αo, and the cation channel TRP melastatin 1 (TRPM1). This system is thought to be common to both the rod-and ON-cone-driven pathways, which control vision under scotopic and photopic conditions, respectively. In this study, we present evidence that the rod pathway is uniquely susceptible to modulation by PKCαat the rod-rod bipolar cell synapse. Decreased production ofDAG(an activator of PKC) by inhibition of PIP 2 (phosphatidylinositol-4,5-bisphosphate) hydrolysis caused depression of the TRPM1 current. Conversely, addition of a DAG analog, 2-acetyl-1-oleoyl-sn-glycerol (OAG), potentiated the current in rod bipolar cells but not in ON-cone bipolar cells. The potentiating effects ofOAGwere absent both in mutant mice that lack PKCα expression and in wild-type mice in which enzymatic activity of PKCα was pharmacologically inhibited. In addition, we found that, like other members of the TRPM subfamily, TRPM1 current is susceptible to voltage-independent inhibition by intracellular magnesium, and that modulation by PKCα relieves this inhibition, as the potentiating effects of OAG are absent in low intracellular magnesium. We conclude that activation of PKCα initiates a modulatory mechanism at the rod-rod bipolar cell synapse whose function is to reduce inhibition of the TRPM1 current by magnesium, thereby increasing the gain of transmission at this synapse.

Original languageEnglish (US)
Pages (from-to)13596-13603
Number of pages8
JournalJournal of Neuroscience
Volume31
Issue number38
DOIs
StatePublished - Sep 21 2011

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Synapses
Magnesium
Night Vision
Color Vision
Vertebrate Photoreceptor Cells
Phosphatidylinositols
GTP-Binding Proteins
Retina
Inhibition (Psychology)
Cations
Hydrolysis
Light
trophoblastin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Relief of mg 2+-dependent inhibition of trpm1 by pkcα at the rod bipolar cell synapse. / Rampino, Melissa Ann F; Nawy, Scott A.

In: Journal of Neuroscience, Vol. 31, No. 38, 21.09.2011, p. 13596-13603.

Research output: Contribution to journalArticle

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