Release of Glutamate and Aspartate from CA1 Synaptosomes: Selective Modulation of Aspartate Release by Ionotropic Glutamate Receptor Ligands

Ming Zhou, Cathleen L. Peterson, Ya‐Bin ‐B Lu, J. Victor Nadler

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Abstract: Synaptosomes prepared from area CA1 of the rat hippocampus were used to determine (a) whether Schaffer collateral‐commissural‐ipsilateral associational terminals release both aspartate and glutamate in a Ca2+‐dependent manner when reuptake of released glutamate is minimal and (b) whether autoreceptor mechanisms described in CA1 or hippocampal slices could reflect direct actions of glutamate receptor ligands on the synaptic terminal. When challenged for 1 min with either 25 mM K+ or 300 µM 4‐aminopyridine, CA1 synaptosomes released both glutamate and aspartate in a Ca2+‐dependent manner. The glutamate/aspartate ratio was ∼5:1 in each case. K+‐evoked glutamate release was unaffected by ligands active at NMDA or (RS)‐α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionate (AMPA) receptors. Unlike glutamate release, the release of aspartate was enhanced by NMDA, and this effect was blocked by d‐2‐amino‐5‐phosphonovalerate (d‐AP5). Kainate selectively depressed and 6‐cyano‐7‐nitroquinoxaline‐2,3‐dione (CNQX) selectively increased the K+‐evoked release of aspartate. AMPA enhanced aspartate release, like the antagonist CNQX. When applied in the presence of diazoxide, which blocks the desensitization of AMPA receptors, AMPA and kainate both depressed aspartate release. These findings support the view that Schaffer collateral‐commissural‐ipsilateral associational terminals release aspartate as well as glutamate and that these two release processes are regulated by different autoreceptor mechanisms.

Original languageEnglish (US)
Pages (from-to)1556-1566
Number of pages11
JournalJournal of Neurochemistry
Volume64
Issue number4
DOIs
StatePublished - Apr 1995

Fingerprint

Ionotropic Glutamate Receptors
Synaptosomes
Aspartic Acid
Glutamic Acid
Modulation
Ligands
6-Cyano-7-nitroquinoxaline-2,3-dione
Autoreceptors
N-Methylaspartate
Diazoxide
Kainic Acid Receptors
Kainic Acid
Presynaptic Terminals
Glutamate Receptors
Rats
Hippocampus

Keywords

  • Aspartate
  • Autoreceptor
  • Glutamate
  • Hippocampus
  • Synaptosomes
  • Transmitter release

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Release of Glutamate and Aspartate from CA1 Synaptosomes : Selective Modulation of Aspartate Release by Ionotropic Glutamate Receptor Ligands. / Zhou, Ming; Peterson, Cathleen L.; Lu, Ya‐Bin ‐B; Nadler, J. Victor.

In: Journal of Neurochemistry, Vol. 64, No. 4, 04.1995, p. 1556-1566.

Research output: Contribution to journalArticle

Zhou, Ming ; Peterson, Cathleen L. ; Lu, Ya‐Bin ‐B ; Nadler, J. Victor. / Release of Glutamate and Aspartate from CA1 Synaptosomes : Selective Modulation of Aspartate Release by Ionotropic Glutamate Receptor Ligands. In: Journal of Neurochemistry. 1995 ; Vol. 64, No. 4. pp. 1556-1566.
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