Relative contribution of porcine reproductive and respiratory syndrome virus open reading frames 2–4 to the induction of protective immunity

Kathryn Kimpston-Burkgren, Ignacio Correas, Fernando A. Osorio, David J Steffen, Asit K Pattnaik, Ying Fang, Hiep L Vu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The minor glycoproteins (GPs) of PRRSV, GP2, GP3, and GP4, form a heterotrimer that is required for viral infectivity, presumably due to its interaction with the key cellular receptor CD163. These 3 GPs are encoded by open reading frames (ORFs) 2a, 3 and 4 (herein referred to as ORFs 2–4), respectively. The goal of this study was to investigate the immunogenicity of the PRRSV-2 minor GPs. Through the use of reverse genetics, a chimeric virus (designated SDFL24) was constructed by replacing ORFs 2–4 of the PRRSV-1 strain SD01-08 with the corresponding genes of the PRRSV-2 strain FL12. While the parental PRRSV strain SD01-08 was not neutralized by convalescent antisera raised against FL12, the chimeric virus SDFL24 gained susceptibility to neutralization by FL12-specific antisera, indicating that viral proteins encoded by ORFs 2–4 are targets of antibody neutralization. When inoculated into pigs, the chimeric virus SDFL24 elicited T-cell responses against peptides derived from FL12 minor GPs, whereas the parental virus SD01-08 did not. After challenge infection with FL12, pigs previously infected with SDFL24 developed robust kinetics of FL12-specific neutralizing antibodies as compared to those previously infected with the parental strain SD01-08. Finally, the pigs recovered from SDFL24 infection were better protected from a subsequent challenge infection with FL12 than those previously infected with SD01-08. Collectively, the results indicate that PRRSV-2 ORFs 2–4 are capable of inducing protective immunity.

Original languageEnglish (US)
Pages (from-to)4408-4413
Number of pages6
JournalVaccine
Volume35
Issue number34
DOIs
StatePublished - Aug 3 2017

Fingerprint

Porcine respiratory and reproductive syndrome virus
Porcine reproductive and respiratory syndrome virus
Open Reading Frames
open reading frames
Immunity
immunity
glycoproteins
Glycoproteins
Viruses
viruses
Swine
neutralization
swine
antiserum
Immune Sera
Infection
infection
Reverse Genetics
viral proteins
Viral Proteins

Keywords

  • Chimeric virus
  • Immunization-challenge experiment
  • Minor glycoproteins
  • PRRSV

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Relative contribution of porcine reproductive and respiratory syndrome virus open reading frames 2–4 to the induction of protective immunity. / Kimpston-Burkgren, Kathryn; Correas, Ignacio; Osorio, Fernando A.; Steffen, David J; Pattnaik, Asit K; Fang, Ying; Vu, Hiep L.

In: Vaccine, Vol. 35, No. 34, 03.08.2017, p. 4408-4413.

Research output: Contribution to journalArticle

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