Abstract

Background: Agriculture workers are exposed to microbial component- and particulate matter-enriched organic dust aerosols. Whereas it is clear that exposure to these aerosols can lead to lung inflammation, it is not known how inflammatory responses are resolved in some individuals while others develop chronic lung disease. Interleukin (IL)-10 is an immunomodulatory cytokine that is recognized as a potent anti-inflammatory and pro-resolving factor. The objective of this study was to determine whether there is a relationship of systemic IL-10 and proinflammatory responses and/or respiratory health effects in humans with prior agriculture exposure. Methods: This is a cross sectional study of 625 veterans with > 2 years of farming experience. Whole blood was stimulated with or without organic dust and measured for IL-6, TNFα and IL-10. Participants underwent spirometry and respiratory symptoms were assessed by questionnaire. Results: We found that baseline IL-10 concentration from the whole blood assay was inversely associated with ∆TNF-α (r = - 0.63) and ∆IL-6 (r = - 0.37) levels. Results remained highly significant in the linear regression model after adjusting for age, sex, BMI, race, education, smoking status, and white blood cell count (∆TNF-α, p < 0.0001; ∆IL-6, p < 0.0001). We found no association between chronic cough (p = 0.18), chronic phlegm (p = 0.31) and chronic bronchitis (p = 0.06) and baseline IL-10 levels using univariate logistic regression models. However, we did find that higher FEV1/FVC was significantly associated with increased baseline IL-10 concentration. Conclusions: Collectively, these studies support a potential role for IL-10 in modulating an inflammatory response and lung function in agriculture-exposed persons.

Original languageEnglish (US)
Article number166
JournalRespiratory Research
Volume19
Issue number1
DOIs
StatePublished - Sep 3 2018

Fingerprint

Agriculture
Interleukin-10
Cytokines
Lung
Interleukin-6
Aerosols
Dust
Linear Models
Logistic Models
Particulate Matter
Spirometry
Chronic Bronchitis
Veterans
Leukocyte Count
Cough
Lung Diseases
Pneumonia
Chronic Disease
Anti-Inflammatory Agents
Cross-Sectional Studies

Keywords

  • Agriculture
  • Airway disease
  • IL-10
  • Organic dust

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

@article{fa687682926041c5abffbc931fdf32a3,
title = "Relationship of systemic IL-10 levels with proinflammatory cytokine responsiveness and lung function in agriculture workers",
abstract = "Background: Agriculture workers are exposed to microbial component- and particulate matter-enriched organic dust aerosols. Whereas it is clear that exposure to these aerosols can lead to lung inflammation, it is not known how inflammatory responses are resolved in some individuals while others develop chronic lung disease. Interleukin (IL)-10 is an immunomodulatory cytokine that is recognized as a potent anti-inflammatory and pro-resolving factor. The objective of this study was to determine whether there is a relationship of systemic IL-10 and proinflammatory responses and/or respiratory health effects in humans with prior agriculture exposure. Methods: This is a cross sectional study of 625 veterans with > 2 years of farming experience. Whole blood was stimulated with or without organic dust and measured for IL-6, TNFα and IL-10. Participants underwent spirometry and respiratory symptoms were assessed by questionnaire. Results: We found that baseline IL-10 concentration from the whole blood assay was inversely associated with ∆TNF-α (r = - 0.63) and ∆IL-6 (r = - 0.37) levels. Results remained highly significant in the linear regression model after adjusting for age, sex, BMI, race, education, smoking status, and white blood cell count (∆TNF-α, p < 0.0001; ∆IL-6, p < 0.0001). We found no association between chronic cough (p = 0.18), chronic phlegm (p = 0.31) and chronic bronchitis (p = 0.06) and baseline IL-10 levels using univariate logistic regression models. However, we did find that higher FEV1/FVC was significantly associated with increased baseline IL-10 concentration. Conclusions: Collectively, these studies support a potential role for IL-10 in modulating an inflammatory response and lung function in agriculture-exposed persons.",
keywords = "Agriculture, Airway disease, IL-10, Organic dust",
author = "LeVan, {Tricia D} and Debra Romberger and Mohammad Siahpush and Grimm, {Brandon L} and Ramos, {Athena K} and Johansson, {Patrik L} and Michaud, {Tzeyu L.} and Heires, {Art J.} and Wyatt, {Todd A} and Poole, {Jill A}",
year = "2018",
month = "9",
day = "3",
doi = "10.1186/s12931-018-0875-z",
language = "English (US)",
volume = "19",
journal = "Respiratory Research",
issn = "1465-9921",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Relationship of systemic IL-10 levels with proinflammatory cytokine responsiveness and lung function in agriculture workers

AU - LeVan, Tricia D

AU - Romberger, Debra

AU - Siahpush, Mohammad

AU - Grimm, Brandon L

AU - Ramos, Athena K

AU - Johansson, Patrik L

AU - Michaud, Tzeyu L.

AU - Heires, Art J.

AU - Wyatt, Todd A

AU - Poole, Jill A

PY - 2018/9/3

Y1 - 2018/9/3

N2 - Background: Agriculture workers are exposed to microbial component- and particulate matter-enriched organic dust aerosols. Whereas it is clear that exposure to these aerosols can lead to lung inflammation, it is not known how inflammatory responses are resolved in some individuals while others develop chronic lung disease. Interleukin (IL)-10 is an immunomodulatory cytokine that is recognized as a potent anti-inflammatory and pro-resolving factor. The objective of this study was to determine whether there is a relationship of systemic IL-10 and proinflammatory responses and/or respiratory health effects in humans with prior agriculture exposure. Methods: This is a cross sectional study of 625 veterans with > 2 years of farming experience. Whole blood was stimulated with or without organic dust and measured for IL-6, TNFα and IL-10. Participants underwent spirometry and respiratory symptoms were assessed by questionnaire. Results: We found that baseline IL-10 concentration from the whole blood assay was inversely associated with ∆TNF-α (r = - 0.63) and ∆IL-6 (r = - 0.37) levels. Results remained highly significant in the linear regression model after adjusting for age, sex, BMI, race, education, smoking status, and white blood cell count (∆TNF-α, p < 0.0001; ∆IL-6, p < 0.0001). We found no association between chronic cough (p = 0.18), chronic phlegm (p = 0.31) and chronic bronchitis (p = 0.06) and baseline IL-10 levels using univariate logistic regression models. However, we did find that higher FEV1/FVC was significantly associated with increased baseline IL-10 concentration. Conclusions: Collectively, these studies support a potential role for IL-10 in modulating an inflammatory response and lung function in agriculture-exposed persons.

AB - Background: Agriculture workers are exposed to microbial component- and particulate matter-enriched organic dust aerosols. Whereas it is clear that exposure to these aerosols can lead to lung inflammation, it is not known how inflammatory responses are resolved in some individuals while others develop chronic lung disease. Interleukin (IL)-10 is an immunomodulatory cytokine that is recognized as a potent anti-inflammatory and pro-resolving factor. The objective of this study was to determine whether there is a relationship of systemic IL-10 and proinflammatory responses and/or respiratory health effects in humans with prior agriculture exposure. Methods: This is a cross sectional study of 625 veterans with > 2 years of farming experience. Whole blood was stimulated with or without organic dust and measured for IL-6, TNFα and IL-10. Participants underwent spirometry and respiratory symptoms were assessed by questionnaire. Results: We found that baseline IL-10 concentration from the whole blood assay was inversely associated with ∆TNF-α (r = - 0.63) and ∆IL-6 (r = - 0.37) levels. Results remained highly significant in the linear regression model after adjusting for age, sex, BMI, race, education, smoking status, and white blood cell count (∆TNF-α, p < 0.0001; ∆IL-6, p < 0.0001). We found no association between chronic cough (p = 0.18), chronic phlegm (p = 0.31) and chronic bronchitis (p = 0.06) and baseline IL-10 levels using univariate logistic regression models. However, we did find that higher FEV1/FVC was significantly associated with increased baseline IL-10 concentration. Conclusions: Collectively, these studies support a potential role for IL-10 in modulating an inflammatory response and lung function in agriculture-exposed persons.

KW - Agriculture

KW - Airway disease

KW - IL-10

KW - Organic dust

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U2 - 10.1186/s12931-018-0875-z

DO - 10.1186/s12931-018-0875-z

M3 - Article

C2 - 30176916

AN - SCOPUS:85052702331

VL - 19

JO - Respiratory Research

JF - Respiratory Research

SN - 1465-9921

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