Relationship of neuropeptide FF receptors with pubertal maturation of gilts

Jennifer F. Thorson, Neely L. Heidorn, Vitaly Ryu, Krzysztof Czaja, Danny J. Nonneman, C. Richard Barb, Gary J. Hausman, Gary A. Rohrer, Ligia D. Prezotto, Richard B. McCosh, Elane C. Wright, Brett R. White, Bradley A. Freking, William T. Oliver, Stanley M. Hileman, Clay A. Lents

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mechanisms governing the timing of puberty in pigs are poorly understood. A genome-wide association study for age at first estrus in pigs identified candidate genes including neuropeptide FF receptor 2 (NPFFR2), which is a putative receptor for RFamide-related peptides (RFRP). RFRP has been shown to negatively regulate secretion of reproductive hormones from hypothalamic and pituitary tissue of pigs in culture. Here, the porcine NPFFR2 gene was further screened and four potentially functional variants were identified to be associated with age at first estrus in pigs (1,288 gilts). The RFRP neurons in the porcine hypothalamus were localized in the paraventricular and dorsomedial nuclei with RFRP fibers in the lateral hypothalamic area. There were marked changes in expression of NPFF receptors in the anterior pituitary gland and hypothalamus of gilts beginning with the peripubertal period. The hypothesis that NPFF receptor function is related to secretion of luteinizing hormone (LH) in gilts was tested with various NPFF receptor ligands. The NPFF receptor antagonist RF9 stimulated a pulse-like release of LH in prepubertal gilts. The putative NPFF receptor agonist RFRP3modestly suppressed LH pulses in ovariectomized (OVX) prepubertal gilts. A porcine-specific RFRP2 failed to have an effect on LH secretion in OVX prepubertal gilts despite its high degree of homology to avian gonadotropin-inhibitory hormone. Results indicate that an RFRP system is present in the pig and that NPFFR2 is important for pubertal onset in gilts. It is not clear if this regulation involves major control of LH secretion or another unknown mechanism.

Original languageEnglish (US)
Article numberbio144998
Pages (from-to)617-634
Number of pages18
JournalBiology of reproduction
Volume96
Issue number3
DOIs
StatePublished - Mar 1 2017

Fingerprint

Swine
Luteinizing Hormone
Estrus
Hypothalamus
Hypothalamic Hormones
Lateral Hypothalamic Area
Mediodorsal Thalamic Nucleus
Anterior Pituitary Gland
Pituitary Hormones
neuropeptide FF receptor
Paraventricular Hypothalamic Nucleus
Genome-Wide Association Study
Puberty
Gonadotropins
Genes
RFamide peptide
Hormones
Ligands
Neurons

Keywords

  • Anterior pituitary
  • Domestic animal reproduction
  • Gene expression
  • Genetics
  • Hypothalamus
  • Luteinizing hormone
  • Neuropeptide receptors
  • Neuropeptides
  • Porcine/pig
  • Puberty

ASJC Scopus subject areas

  • Cell Biology

Cite this

Thorson, J. F., Heidorn, N. L., Ryu, V., Czaja, K., Nonneman, D. J., Barb, C. R., ... Lents, C. A. (2017). Relationship of neuropeptide FF receptors with pubertal maturation of gilts. Biology of reproduction, 96(3), 617-634. [bio144998]. https://doi.org/10.1095/biolreprod.116.144998

Relationship of neuropeptide FF receptors with pubertal maturation of gilts. / Thorson, Jennifer F.; Heidorn, Neely L.; Ryu, Vitaly; Czaja, Krzysztof; Nonneman, Danny J.; Barb, C. Richard; Hausman, Gary J.; Rohrer, Gary A.; Prezotto, Ligia D.; McCosh, Richard B.; Wright, Elane C.; White, Brett R.; Freking, Bradley A.; Oliver, William T.; Hileman, Stanley M.; Lents, Clay A.

In: Biology of reproduction, Vol. 96, No. 3, bio144998, 01.03.2017, p. 617-634.

Research output: Contribution to journalArticle

Thorson, JF, Heidorn, NL, Ryu, V, Czaja, K, Nonneman, DJ, Barb, CR, Hausman, GJ, Rohrer, GA, Prezotto, LD, McCosh, RB, Wright, EC, White, BR, Freking, BA, Oliver, WT, Hileman, SM & Lents, CA 2017, 'Relationship of neuropeptide FF receptors with pubertal maturation of gilts', Biology of reproduction, vol. 96, no. 3, bio144998, pp. 617-634. https://doi.org/10.1095/biolreprod.116.144998
Thorson JF, Heidorn NL, Ryu V, Czaja K, Nonneman DJ, Barb CR et al. Relationship of neuropeptide FF receptors with pubertal maturation of gilts. Biology of reproduction. 2017 Mar 1;96(3):617-634. bio144998. https://doi.org/10.1095/biolreprod.116.144998
Thorson, Jennifer F. ; Heidorn, Neely L. ; Ryu, Vitaly ; Czaja, Krzysztof ; Nonneman, Danny J. ; Barb, C. Richard ; Hausman, Gary J. ; Rohrer, Gary A. ; Prezotto, Ligia D. ; McCosh, Richard B. ; Wright, Elane C. ; White, Brett R. ; Freking, Bradley A. ; Oliver, William T. ; Hileman, Stanley M. ; Lents, Clay A. / Relationship of neuropeptide FF receptors with pubertal maturation of gilts. In: Biology of reproduction. 2017 ; Vol. 96, No. 3. pp. 617-634.
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