Relationship between the cervical microbiome, HIV Status, and precancerous lesions

Cameron Klein, Daniela Gonzalez, Kandali Samwel, Crispin Kahesa, Julius Mwaiselage, Nirosh Aluthge, Samodha Fernando, John T. West, Charles Wood, Peter C. Angeletti

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Nearly all cervical cancers are causally associated with human papillomavirus (HPV). The burden of HPV-associated dysplasias in sub-Saharan Africa is influenced by HIV. To investigate the role of the bacterial microbiome in cervical dysplasia, cytobrush samples were collected directly from cervical lesions of 144 Tanzanian women. The V4 hypervariable region of the 16S rRNA gene was amplified and deep sequenced. Alpha diversity metrics (Chao1, PD whole tree, and operational taxonomic unit [OTU] estimates) displayed significantly higher bacterial richness in HIV-positive patients (P = 0.01) than in HIV-negative patients. In HIV-positive patients, there was higher bacterial richness in patients with high-grade squamous intraepithelial lesions (HSIL) (P = 0.13) than those without lesions. The most abundant OTUs associated with high-grade squamous intraepithelial lesions were Mycoplasmatales, Pseudomonadales, and Staphylococcus. We suggest that a chronic mycoplasma infection of the cervix may contribute to HPV-dependent dysplasia by sustained inflammatory signals. IMPORTANCE HPV is known to be the causal agent in the majority of cervical cancers. However, the role of the cervical bacterial microbiome in cervical cancer is not clear. To investigate that possibility, we collected cervical cytobrush samples from 144 Tanzanian women and performed deep sequencing of bacterial 16S rRNA genes. We found that HIV-positive patients had greater bacterial richness (P = 0.01) than HIV-negative patients. We also observed that women with high-grade squamous intraepithelial lesions (HSIL) had greater cervical bacterial diversity than women with cytologically normal cervices. Data from our precise sampling of cervical lesions leads us to propose that Mycoplasma contributes to a cervical microbiome status that promotes HPV-related cervical lesions. These results suggest a greater influence of the bacterial microbiota on the outcome of HPV infection than previously thought.

Original languageEnglish (US)
Article numbere02785-18
JournalmBio
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Microbiota
HIV
Uterine Cervical Neoplasms
rRNA Genes
Cervix Uteri
Mycoplasmatales
Uterine Cervical Dysplasia
Mycoplasma Infections
High-Throughput Nucleotide Sequencing
Papillomavirus Infections
Mycoplasma
Africa South of the Sahara
Staphylococcus
Squamous Intraepithelial Lesions of the Cervix

Keywords

  • 16S RNA
  • Cervical cancer
  • Deep sequencing
  • Human immunodeficiency virus
  • Human papillomavirus
  • Microbiome

ASJC Scopus subject areas

  • Microbiology
  • Virology

Cite this

Klein, C., Gonzalez, D., Samwel, K., Kahesa, C., Mwaiselage, J., Aluthge, N., ... Angeletti, P. C. (2019). Relationship between the cervical microbiome, HIV Status, and precancerous lesions. mBio, 10(1), [e02785-18]. https://doi.org/10.1128/mBio.02785-18

Relationship between the cervical microbiome, HIV Status, and precancerous lesions. / Klein, Cameron; Gonzalez, Daniela; Samwel, Kandali; Kahesa, Crispin; Mwaiselage, Julius; Aluthge, Nirosh; Fernando, Samodha; West, John T.; Wood, Charles; Angeletti, Peter C.

In: mBio, Vol. 10, No. 1, e02785-18, 01.01.2019.

Research output: Contribution to journalArticle

Klein C, Gonzalez D, Samwel K, Kahesa C, Mwaiselage J, Aluthge N et al. Relationship between the cervical microbiome, HIV Status, and precancerous lesions. mBio. 2019 Jan 1;10(1). e02785-18. https://doi.org/10.1128/mBio.02785-18
Klein, Cameron ; Gonzalez, Daniela ; Samwel, Kandali ; Kahesa, Crispin ; Mwaiselage, Julius ; Aluthge, Nirosh ; Fernando, Samodha ; West, John T. ; Wood, Charles ; Angeletti, Peter C. / Relationship between the cervical microbiome, HIV Status, and precancerous lesions. In: mBio. 2019 ; Vol. 10, No. 1.
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