Relation between BK-α/β4-mediated potassium secretion and ENaC-mediated sodium reabsorption

Donghai Wen, Ryan J. Cornelius, Dianelys Rivero-Hernandez, Yang Yuan, Huaqing Li, Alan M. Weinstein, Steven C. Sansom

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The large-conductance, calcium-activated BK-α/β4 potassium channel, localized to the intercalated cells of the distal nephron, mediates potassium secretion during high-potassium, alkaline diets. Here we determine whether BK-α/β4-mediated potassium transport is dependent on epithelial sodium channel (ENaC)-mediated sodium reabsorption. We maximized sodium-potassium exchange in the distal nephron by feeding mice a low-sodium, high-potassium diet. Wild-type and BK-β4 knockout mice were maintained on a low-sodium, high-potassium, alkaline diet or a low-sodium, high-potassium, acidic diet for 7-10 days. Wild-type mice maintained potassium homeostasis on the alkaline, but not acid, diet. BK-β4 knockout mice could not maintain potassium homeostasis on either diet. During the last 12 h of diet, wild-type mice on either a regular, alkaline, or an acid diet, or knockout mice on an alkaline diet, were administered amiloride (an ENaC inhibitor). Amiloride enhanced sodium excretion in all wild-type and knockout groups to similar values; however, amiloride diminished potassium excretion by 59% in wild-type but only by 33% in knockout mice on an alkaline diet. Similarly, amiloride decreased the trans-tubular potassium gradient by 68% in wild-type but only by 42% in knockout mice on an alkaline diet. Amiloride treatment equally enhanced sodium excretion and diminished potassium secretion in knockout mice on an alkaline diet and wild-type mice on an acid diet. Thus, the enhanced effect of amiloride on potassium secretion in wild-type compared to knockout mice on the alkaline diet clarify a BK- α/β4-mediated potassium secretory pathway in intercalated cells driven by ENaC-mediated sodium reabsorption linked to bicarbonate secretion.

Original languageEnglish (US)
Pages (from-to)139-145
Number of pages7
JournalKidney International
Volume86
Issue number1
DOIs
StatePublished - Jul 2014

Fingerprint

Potassium
Sodium
Diet
Amiloride
Knockout Mice
Nephrons
Acids
Homeostasis
Epithelial Sodium Channels
Secretory Pathway
Potassium Channels
Bicarbonates
Calcium

Keywords

  • BK-α/β4
  • ENaC
  • K secretion
  • Na reabsorption
  • TTKG
  • math modeling

ASJC Scopus subject areas

  • Nephrology

Cite this

Wen, D., Cornelius, R. J., Rivero-Hernandez, D., Yuan, Y., Li, H., Weinstein, A. M., & Sansom, S. C. (2014). Relation between BK-α/β4-mediated potassium secretion and ENaC-mediated sodium reabsorption. Kidney International, 86(1), 139-145. https://doi.org/10.1038/ki.2014.14

Relation between BK-α/β4-mediated potassium secretion and ENaC-mediated sodium reabsorption. / Wen, Donghai; Cornelius, Ryan J.; Rivero-Hernandez, Dianelys; Yuan, Yang; Li, Huaqing; Weinstein, Alan M.; Sansom, Steven C.

In: Kidney International, Vol. 86, No. 1, 07.2014, p. 139-145.

Research output: Contribution to journalArticle

Wen, D, Cornelius, RJ, Rivero-Hernandez, D, Yuan, Y, Li, H, Weinstein, AM & Sansom, SC 2014, 'Relation between BK-α/β4-mediated potassium secretion and ENaC-mediated sodium reabsorption', Kidney International, vol. 86, no. 1, pp. 139-145. https://doi.org/10.1038/ki.2014.14
Wen D, Cornelius RJ, Rivero-Hernandez D, Yuan Y, Li H, Weinstein AM et al. Relation between BK-α/β4-mediated potassium secretion and ENaC-mediated sodium reabsorption. Kidney International. 2014 Jul;86(1):139-145. https://doi.org/10.1038/ki.2014.14
Wen, Donghai ; Cornelius, Ryan J. ; Rivero-Hernandez, Dianelys ; Yuan, Yang ; Li, Huaqing ; Weinstein, Alan M. ; Sansom, Steven C. / Relation between BK-α/β4-mediated potassium secretion and ENaC-mediated sodium reabsorption. In: Kidney International. 2014 ; Vol. 86, No. 1. pp. 139-145.
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