Reintroduction of a normal retinoblastoma gene into retinoblastoma and osteosarcoma cells inhibits the replication associated function of SV40 large T antigen.

E. Uzvolgyi, M. Classon, M. Henriksson, H. J. Huang, L. Szekely, W. H. Lee, G. Klein, J. Sumegi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The product of the retinoblastoma (Rb) gene can form complexes with the transforming proteins of small DNA tumor viruses, including SV40 large T antigen (Tag), adenovirus E1A, and the human papilloma virus E7. The strong correlation between their ability to transform and their ability to bind Rb protein suggests that these oncoproteins exert their effect through blocking the Rb function. SV40 Tag causes oncogenic cell transformation of rodent cells, and it is also required for viral DNA replication. In this paper, we investigated the effect of the Rb protein on the SV40 replication associated function of Tag. We present evidence suggesting that the complex formation between Rb and Tag interferes with the viral DNA replication. In Y79 retinoblastoma and Saos-2 osteosarcoma cells, which lack functional Rb protein, a SV40 based plasmid vector, pSVEpR4, replicates well. In the same cells reconstituted for Rb expression with an intact Rb gene introduced by retroviral mediated gene transfer, pSVEpR4 replicates to a considerably lower level. The inhibitory effect of Rb protein was surmounted by increasing the intracellular level of Tag. Increasing amounts of Tag in wild-type Rb negative Y79 cells had virtually no effect on SV40 replication. Furthermore, the overexpression of Tag in Rb reconstituted Y79 cells did not alter the growth rate of the cells. These data suggest that Rb protein interacts with Tag and modulates its ability to promote SV40 DNA replication.

Original languageEnglish (US)
Pages (from-to)297-303
Number of pages7
JournalCell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
Volume2
Issue number6
StatePublished - Jun 1991

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Retinoblastoma Genes
Polyomavirus Transforming Antigens
Retinoblastoma
Viral Tumor Antigens
Osteosarcoma
Retinoblastoma Protein
DNA Replication
Viral DNA
Papillomaviridae
DNA Tumor Viruses
Oncogene Proteins
Adenoviridae
Rodentia
Plasmids
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Reintroduction of a normal retinoblastoma gene into retinoblastoma and osteosarcoma cells inhibits the replication associated function of SV40 large T antigen. / Uzvolgyi, E.; Classon, M.; Henriksson, M.; Huang, H. J.; Szekely, L.; Lee, W. H.; Klein, G.; Sumegi, J.

In: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 2, No. 6, 06.1991, p. 297-303.

Research output: Contribution to journalArticle

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AU - Klein, G.

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