Regulatory regions in DNA

promoters, enhancers, silencers, and insulators.

Research output: Contribution to journalReview article

46 Citations (Scopus)

Abstract

One of the mechanisms through which protein levels in the cell are controlled is through transcriptional regulation. Certain regions, called cis-regulatory elements, on the DNA are footprints for the trans-acting proteins involved in transcription, either for the positioning of the basic transcriptional machinery or for the regulation - in simple terms turn on or turn off - thereof. The basic transcriptional machinery is DNA-dependent RNA polymerase (RNAP) which synthesizes various types of RNA and core promoters on the DNA are used to position the RNAP. Other nearby regions will regulate the transcription: in prokaryotic organisms operators are involved; in eukaryotic organisms, proximal promoter regions, enhancers, silencers, and insulators are present. This chapter will describe the various DNA regions involved in transcription and transcriptional regulation.

Original languageEnglish (US)
Pages (from-to)33-42
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume674
StatePublished - Jan 1 2010

Fingerprint

Nucleic Acid Regulatory Sequences
DNA-Directed RNA Polymerases
DNA Footprinting
DNA
Genetic Promoter Regions
Proteins
RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Regulatory regions in DNA : promoters, enhancers, silencers, and insulators. / Riethoven, Jean-Jack M.

In: Methods in molecular biology (Clifton, N.J.), Vol. 674, 01.01.2010, p. 33-42.

Research output: Contribution to journalReview article

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