Regulatory Effects of Cell Density on the Binding of Transforming Growth Factor β, Epidermal Growth Factor, Platelet-Derived Growth Factor, and Fibroblast Growth Factor

A Angie Rizzino, Peter Kazakoff, Eric Ruff, Charles Kuszynski, John Nebelsick

Research output: Contribution to journalArticle

Abstract

The work described in this paper demonstrates that the cellular binding of transforming growth factor β, epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor is reduced as cell density is increased. The reduction in transforming growth factor β binding was observed in five different cell lines. Examination of several of the cell lines, under conditions where transforming growth factor β binding is reduced, revealed that epidermal growth factor binding, platelet-derived growth factor binding, and fibroblast growth factor binding are also reduced. In the case of NRK-49F cells, the reduction in transforming growth factor β binding results from a decrease in the number of high-affinity receptors and not from a change in receptor affinity. Similarly, it was determined that the reduction in epidermal growth factor binding is due to a selective reduction in the high-affinity receptors for epidermal growth factor. Overall, the data suggest that the effect of cell density on growth factor binding, which we refer to as density-induced down regulation of growth factor receptors, differs both from down regulation induced by a specific growth factor and from receptor transmodulation.

Original languageEnglish (US)
Pages (from-to)4256-4260
Number of pages5
JournalCancer Research
Volume48
Issue number15
StatePublished - 1988

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Fibroblast Growth Factors
Platelet-Derived Growth Factor
Transforming Growth Factors
Epidermal Growth Factor
Cell Count
Growth Factor Receptors
Down-Regulation
Cell Line
Intercellular Signaling Peptides and Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Regulatory Effects of Cell Density on the Binding of Transforming Growth Factor β, Epidermal Growth Factor, Platelet-Derived Growth Factor, and Fibroblast Growth Factor. / Rizzino, A Angie; Kazakoff, Peter; Ruff, Eric; Kuszynski, Charles; Nebelsick, John.

In: Cancer Research, Vol. 48, No. 15, 1988, p. 4256-4260.

Research output: Contribution to journalArticle

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N2 - The work described in this paper demonstrates that the cellular binding of transforming growth factor β, epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor is reduced as cell density is increased. The reduction in transforming growth factor β binding was observed in five different cell lines. Examination of several of the cell lines, under conditions where transforming growth factor β binding is reduced, revealed that epidermal growth factor binding, platelet-derived growth factor binding, and fibroblast growth factor binding are also reduced. In the case of NRK-49F cells, the reduction in transforming growth factor β binding results from a decrease in the number of high-affinity receptors and not from a change in receptor affinity. Similarly, it was determined that the reduction in epidermal growth factor binding is due to a selective reduction in the high-affinity receptors for epidermal growth factor. Overall, the data suggest that the effect of cell density on growth factor binding, which we refer to as density-induced down regulation of growth factor receptors, differs both from down regulation induced by a specific growth factor and from receptor transmodulation.

AB - The work described in this paper demonstrates that the cellular binding of transforming growth factor β, epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor is reduced as cell density is increased. The reduction in transforming growth factor β binding was observed in five different cell lines. Examination of several of the cell lines, under conditions where transforming growth factor β binding is reduced, revealed that epidermal growth factor binding, platelet-derived growth factor binding, and fibroblast growth factor binding are also reduced. In the case of NRK-49F cells, the reduction in transforming growth factor β binding results from a decrease in the number of high-affinity receptors and not from a change in receptor affinity. Similarly, it was determined that the reduction in epidermal growth factor binding is due to a selective reduction in the high-affinity receptors for epidermal growth factor. Overall, the data suggest that the effect of cell density on growth factor binding, which we refer to as density-induced down regulation of growth factor receptors, differs both from down regulation induced by a specific growth factor and from receptor transmodulation.

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