Regulation of vascular endothelial growth factor expression in human colon carcinoma cells by cell density

Aaryan N. Koura, Wenbiao Liu, Yasuhiko Kitadai, Rakesh K Singh, Robert Radinsky, Lee M. Ellis

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

To determine the effect of cell density on vascular endothelial growth factor (VEGF) expression and the mechanism of this effect, four human colon cancer cell lines were grown as sparse or confluent monolayers or as spheroids. VEGF mRNA increased >2-fold in cells grown as confluent monolayers or spheroids compared with cells grown as sparse monolayers. Semiquantitative reverse transcription-PCR demonstrated a 2-fold increase in the larger VEGF mRNA isoform (189 bp) in confluent cells. Sparse cells grown in conditioned medium from confluent cells demonstrated a >2-fold increase in VEGF mRNA. These data suggest that VEGF expression may be regulated by an unidentified soluble factor.

Original languageEnglish (US)
Pages (from-to)3891-3894
Number of pages4
JournalCancer Research
Volume56
Issue number17
StatePublished - Sep 1 1996

Fingerprint

Vascular Endothelial Growth Factor A
Colon
Cell Count
Carcinoma
RNA Isoforms
Messenger RNA
Conditioned Culture Medium
Colonic Neoplasms
Reverse Transcription
Cell Line
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Koura, A. N., Liu, W., Kitadai, Y., Singh, R. K., Radinsky, R., & Ellis, L. M. (1996). Regulation of vascular endothelial growth factor expression in human colon carcinoma cells by cell density. Cancer Research, 56(17), 3891-3894.

Regulation of vascular endothelial growth factor expression in human colon carcinoma cells by cell density. / Koura, Aaryan N.; Liu, Wenbiao; Kitadai, Yasuhiko; Singh, Rakesh K; Radinsky, Robert; Ellis, Lee M.

In: Cancer Research, Vol. 56, No. 17, 01.09.1996, p. 3891-3894.

Research output: Contribution to journalArticle

Koura, AN, Liu, W, Kitadai, Y, Singh, RK, Radinsky, R & Ellis, LM 1996, 'Regulation of vascular endothelial growth factor expression in human colon carcinoma cells by cell density', Cancer Research, vol. 56, no. 17, pp. 3891-3894.
Koura, Aaryan N. ; Liu, Wenbiao ; Kitadai, Yasuhiko ; Singh, Rakesh K ; Radinsky, Robert ; Ellis, Lee M. / Regulation of vascular endothelial growth factor expression in human colon carcinoma cells by cell density. In: Cancer Research. 1996 ; Vol. 56, No. 17. pp. 3891-3894.
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