Regulation of Transcription of Compatible Solute Transporters by the General Stress Sigma Factor, σB, in Listeria monocytogenes

Mehmet Sevket Cetin, Chaomei Zhang, Robert W. Hutkins, Andrew K. Benson

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Listeria monocytogenes is well known for its durable physiological characteristics, which allow the organism to grow at low temperature and pH and high osmolarity. Growth under high osmolarity depends on the accumulation of compatible solutes, among which glycine betaine and carnitine are the preferred solutes for this organism. Three different transport systems, Gbu, BetL, and OpuC, have been identified in L. monocytogenes which serve to scavenge the preferred compatible solutes. The general stress response regulator σ B has been shown to play an important role in osmotic adaptation in L. monocytogenes, presumably by directing transcription from one or more of the solute transport genes. In the studies presented here, we have used primer extension analyses to identify the promoter elements responsible for transcription of the opuC, gbuA, and betL genes. All three genes are osmotically inducible to some degree. betL is transcribed from a σ B-independent promoter, while gbuA is transcribed from dual promoters, one of which is σB dependent. opuC is transcribed exclusively from a σB-dependent promoter. The betL promoter is similar in sequence to the σB-independent gbuAP1 promoter. Kinetic analysis of transcript accumulation after osmotic upshift demonstrated that σB-dependent transcripts from gbuAP2 and sigB accumulate for an extended period after upshift, suggesting that σB activity may provide a mechanism for sustained high-level expression during osmotic challenge. In contrast to osmotic upshift, expression from the σB-dependent opuC and gbuAP2 promoters after temperature upshift and ethanol stress was minimal, suggesting that additional mechanisms may also participate in regulating transcription from these σ B-dependent promoters.

Original languageEnglish (US)
Pages (from-to)794-802
Number of pages9
JournalJournal of bacteriology
Volume186
Issue number3
DOIs
StatePublished - Feb 1 2004

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Sigma Factor
Listeria monocytogenes
Osmolar Concentration
Genes
Betaine
Temperature
Carnitine
Ethanol
Growth

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Regulation of Transcription of Compatible Solute Transporters by the General Stress Sigma Factor, σB, in Listeria monocytogenes. / Cetin, Mehmet Sevket; Zhang, Chaomei; Hutkins, Robert W.; Benson, Andrew K.

In: Journal of bacteriology, Vol. 186, No. 3, 01.02.2004, p. 794-802.

Research output: Contribution to journalArticle

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