Regulation of the Escherichia coli sheA gene and characterization of its encoded hemolytic activity

Sandra V. Fernández, Jun Xing, Vivek Kapur, Stephen J. Libby, Raul G Barletta, Rodney A Moxley

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Escherichia coli K-12 carries the cryptic hemolysin gene sheA which is under the control of positive and negative transcriptional regulators. The objectives of the present study were to further analyze the regulation of the sheA gene in E. coli, to compare the sheA genes from E. coli K-12 and a pathogenic E. coli strain, and to characterize the SheA hemolytic activity. Northern blot analysis demonstrated that the transcriptional regulator SlyA activates the E. coli K-12 sheA gene. The main transcriptional start site of the sheA gene was 56 nucleotides upstream from the start codon as determined by primer extension analysis. The sheA genes from E. coli K-12 and a pathogenic E. coli strain were identical. SheA hemolytic activity was cell associated and Ca2+ independent. Copyright (C) 1998 Federation of European Microbiological Societies.

Original languageEnglish (US)
Pages (from-to)85-90
Number of pages6
JournalFEMS Microbiology Letters
Volume168
Issue number1
DOIs
StatePublished - Nov 1 1998

Fingerprint

Escherichia coli
Genes
Hemolysin Proteins
Initiator Codon
Northern Blotting
Nucleotides

Keywords

  • Hemolysin
  • SheA
  • SlyA

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Regulation of the Escherichia coli sheA gene and characterization of its encoded hemolytic activity. / Fernández, Sandra V.; Xing, Jun; Kapur, Vivek; Libby, Stephen J.; Barletta, Raul G; Moxley, Rodney A.

In: FEMS Microbiology Letters, Vol. 168, No. 1, 01.11.1998, p. 85-90.

Research output: Contribution to journalArticle

Fernández, Sandra V. ; Xing, Jun ; Kapur, Vivek ; Libby, Stephen J. ; Barletta, Raul G ; Moxley, Rodney A. / Regulation of the Escherichia coli sheA gene and characterization of its encoded hemolytic activity. In: FEMS Microbiology Letters. 1998 ; Vol. 168, No. 1. pp. 85-90.
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